Data from: Chondroitin sulfate disaccharides modified the structure and function of the murine gut microbiome under healthy and stressed conditions

Chondroitin sulfate (CS) has been widely used for medical and nutraceutical purposes due to its roles in maintaining tissue structural integrity. We investigated if CS disaccharides may act as a bioactive compound and modulate gut microbial composition in mice. Our data show that CS disaccharides supplementation for 16 days significantly reduced blood LPS in the mice experiencing exhaustive exercise stress. CS disaccharides partially restored total fecal short-chain fatty acids from the level significantly repressed in mice under the stress. Our findings demonstrated that CS was likely butyrogenic and resulted in a significant increase in fecal butyrate concentration. CS disaccharides had a profound impact on gut microbial composition, affecting the abundance of 13.6% and 7.3% Operational Taxonomic Units in fecal microbial communities in healthy and stressed mice, respectively. CS disaccharides reduced the prevalence of inflammatory Proteobacteria. Together, our findings demonstrated that CS may ameliorate stress-induced intestinal inflammation. Furthermore, CS significantly increased intestinal Bacteroides acidifaciens population, indirectly exerting its immunomodulatory effect on the intestine. CS disaccharides had a significant impact on a broad range of biological pathways under stressed condition, such as ABC transporters, two-component systems, and carbohydrate metabolism. Our results will facilitate the development of CS as a bioactive nutraceutical.

Genomics and Genetics

Data from: The Majority of Genotypes of the Virulence Gene inlA Are Intact among Natural Watershed Isolates of Listeria monocytogenes from the Central California Coast

Internalin A is an essential virulence gene involved in the uptake of the foodborne pathogen Listeria monocytogenes into host cells. It is intact in clinical strains and often truncated due to Premature Stop Codons (PMSCs) in isolates from processed foods and processing facilities. Less information is known about environmental isolates. We sequenced the inlA alleles and did Multi Locus Variable Number Tandem Repeat Analysis (MLVA) on 112 L. monocytogenes isolates from a 3-year period from naturally contaminated watersheds near a leafy green growing area in Central California.

Genomics and Genetics

NEWT: National Extension Web-mapping Tool

National Extension Web-mapping Tool (or NEWT), is the key in efforts to make spatial data available within the Cooperative Extension System. NEWT requires no GIS experience to use. NEWT provides access to relevant spatial data at a variety of scales (national, state, county) in useful formats (maps, tables, graphs),

Agricultural Products

USDA Dietary Guidelines Sentiment Analysis

This dataset has all documents, the text and the pdf files as well as the code that was used to carry out the sentiment analysis on USDA DietaryGuidelines. The scope of the project and the resulting dataset uploaded here is carrying out the sentiment analysis on USDA dietary guidelines from 1980 till 2015 (released every 5 years). The motivation behind this project was the fact that recommendations regarding the different nutrients have changed over the years.

Ag Data Commons

National Animal Nutrition Program (NANP) Feed Composition Database: Beef

Feed and Ingredient Information is presented as Tables of chemical composition and nutritional value for an extensive list of feed nutrient constituents. The tables include Nutrient, Mean, Number of Samples (n), Standard Deviation (SD), Coefficient of Variation (CV), and Min / Max values for Energy…

Ag Data Commons

Research, Education, and Economics Information System (REEIS)

The Research, Education, and Economics Information System (REEIS) is a source of information on the research, education and extension programs, projects and activities of the U. S. Department of Agriculture (USDA) National Institute of Food and Agriculture (NIFA), the USDA Forest Service, the USDA National Agricultural Statistics Service, the U. S. Patent and Trademark Office, U. S. Census Bureau, and the U. S. National Science Foundation. The system enables users to measure the impact and effectiveness of research, extension and education programs based on data related to agricultural research; forestry research; students, faculty and degrees related to agriculture; USDA partner institution snapshots; Food and nutrition research; 4-H programs; and agricultural snapshots of each state. Internet links to related agencies, institutions, and data bases are also included.

Ag Data Commons