U.S. flag

An official website of the United States government

Data from: Chondroitin sulfate disaccharides modified the structure and function of the murine gut microbiome under healthy and stressed conditions

    Chondroitin sulfate (CS) has been widely used for medical and nutraceutical purposes due to its roles in maintaining tissue structural integrity. We investigated if CS disaccharides may act as a bioactive compound and modulate gut microbial composition in mice. Our data show that CS disaccharides supplementation for 16 days significantly reduced blood LPS in the mice experiencing exhaustive exercise stress. CS disaccharides partially restored total fecal short-chain fatty acids from the level significantly repressed in mice under the stress. Our findings demonstrated that CS was likely butyrogenic and resulted in a significant increase in fecal butyrate concentration. CS disaccharides had a profound impact on gut microbial composition, affecting the abundance of 13.6% and 7.3% Operational Taxonomic Units in fecal microbial communities in healthy and stressed mice, respectively. CS disaccharides reduced the prevalence of inflammatory Proteobacteria. Together, our findings demonstrated that CS may ameliorate stress-induced intestinal inflammation. Furthermore, CS significantly increased intestinal *Bacteroides acidifaciens* population, indirectly exerting its immunomodulatory effect on the intestine. CS disaccharides had a significant impact on a broad range of biological pathways under stressed condition, such as ABC transporters, two-component systems, and carbohydrate metabolism. Our results will facilitate the development of CS as a bioactive nutraceutical.

    Data from: The Majority of Genotypes of the Virulence Gene inlA Are Intact among Natural Watershed Isolates of Listeria monocytogenes from the Central California Coast

      Internalin A is an essential virulence gene involved in the uptake of the foodborne pathogen *Listeria monocytogenes* into host cells. It is intact in clinical strains and often truncated due to Premature Stop Codons (PMSCs) in isolates from processed foods and processing facilities. Less information is known about environmental isolates. We sequenced the inlA alleles and did Multi Locus Variable Number Tandem Repeat Analysis (MLVA) on 112 *L. monocytogenes* isolates from a 3-year period from naturally contaminated watersheds near a leafy green growing area in Central California.

      NEWT: National Extension Web-mapping Tool

        National Extension Web-mapping Tool (or NEWT), is the key in efforts to make spatial data available within the Cooperative Extension System. NEWT requires no GIS experience to use. NEWT provides access to relevant spatial data at a variety of scales (national, state, county) in useful formats (maps, tables, graphs),

        USDA Dietary Guidelines Sentiment Analysis

          This dataset has all documents, the text and the pdf files as well as the code that was used to carry out the sentiment analysis on USDA DietaryGuidelines. The scope of the project and the resulting dataset uploaded here is carrying out the sentiment analysis on USDA dietary guidelines from 1980 till 2015 (released every 5 years). The motivation behind this project was the fact that recommendations regarding the different nutrients have changed over the years.

          National Animal Nutrition Program (NANP) Modeling: Animal Performance Information

            This page allows you to download data from the National Animal Nutrition Program animal performance data repository directly into an Excel file. Component data sets: [NRC Dairy Report](https://animalnutrition.org/node/12); [Lofgreen Garrett](https://animalnutrition.org/node/13); [Environmental Stress](https://animalnutrition.org/node/27): [to follow]; [Beef Digestiblity](https://animalnutrition.org/beef-digestibility): [to follow]; [USDA Beltsville Agricultural Research Center](https://animalnutrition.org/usda-barc).