Watermelon (Citrullus lanatus) and other cucurbits are important crops grown in Guatemala for local consumption and export. Four samplings of crowns, peduncles, and/or leaves of symptomatic plants were made in March and April 2015. Total RNA was extracted from symptomatic plant tissue and tested by RT-PCR for SqVYV, CYSDV, PRSV, and/or begomoviruses. This is the first report of SqVYV infecting watermelon in Central America.
Data from: Genome Sequence of the Thermotolerant Foodborne Pathogen Salmonella enterica Serovar Senftenberg ATCC 43845 and Phylogenetic Analysis of Loci Encoding Increased Protein Quality Control Mechanisms
Salmonella enterica subsp. enterica bacteria are important foodborne pathogens with major economic impact. Some isolates exhibit increased heat tolerance, a concern for food safety. Analysis of a finished-quality genome sequence of an isolate commonly used in heat resistance studies, S. enterica subsp. enterica serovar Senftenberg…
Data from: Draft genome sequences of eight streptogramin-resistant Enterococcus species isolates from animal and environmental sources in the United States
Draft genome sequences of five Enterococcus faecium, two Enterococcus hirae, and one Enterococcus gallinarum from enviromental sources and chicken carcass rinsates. Isolates were selected for their resistance to the streptogramin antibiotic, Quinupristin-Dalfopristin and were all collected in the United States between 2001 and 2004. Antimicrobial resistance genes were identified conferring resistance to the macrolide-lincosamide-streptogramins, aminoglycosides, tetracycline, beta-lactams, and glycopeptides.
Data from: Sporobolus stapfianus: Insights into desiccation tolerance in the resurrection grasses from linking transcriptomics to metabolomics
Non-targeted metabolomics, combined with transcriptomics via a NimbleGen array platform, are used to study how gene expression and metabolite profiles can be linked to generate a more detailed mechanistic appreciation of the cellular response to both desiccation and rehydration in the C4 resurrection grass, Sporobolus stapfianus Gandoger, as a member of a group of important forage grasses.
To study the impact of wheat streak mosaic virus on global gene expression in wheat curl mite, we generated a de novo transcriptome assembly using 50 x 50 paired end reads from the Illumina HiSeq 2500. Reads were assembled using Trinity (version 2.0.6) and contigs greater than 200 nt were retained. All assembled transcripts were annotated using the Trinotate pipeline using blastp searches against the Swiss-prot/Uni-Prot database, blastx searches against the Swiss-prot/Uni-Prot databases, HMM searches against the Pfam-A database, blastp searches against the non-redundant protein database, and signalP and tmHMM predictions. To reduce noise from low abundance transcripts not well supported by the data, we filtered the assembly to retain only those transcripts with TPM values >=0.5.
Data from: Phased Genotyping-by-Sequencing Enhances Analysis of Genetic Diversity and Reveals Divergent Copy Number Variants in Maize
This study supports the notion that genotyping-by-sequencing (GBS) can be tailored to particular aims, and using Zea mays results indicate that large samples of unknown pedigree can be genotyped to obtain complete and accurate GBS data. A GBS procedure was established that produces high call rates per marker (>85%) with accuracy exceeding 99.4%.
Data from: Rerouting of carbon flux in a glycogen mutant of cyanobacteria assessed via isotopically non‐stationary 13C metabolic flux analysis
Isotopically non‐stationary 13C metabolic flux analysis (INST‐13C‐MFA) is performed to analyze rerouting of carbon in a glycogen synthase deficient mutant strain (glgA‐I glgA‐II) of the model cyanobacterium Synechococcus sp. PCC 7002.
Data from: Knockdown of a metathoracic scent gland desaturase enhances the production of (E)‐4‐oxo‐2‐hexenal and suppresses female sexual attractiveness in the plant bug Adelphocoris suturalis
This study isolates and characterizes two new desaturase‐like genes, termed Asutdes1 and Asutdes2, from Adelphocoris suturalis, an important agricultural pest in China.
Transcriptomes were assembled de novo from pools of adult aphids that were feeding on sorghum and switchgrass. Reads from all replicates were pooled, normalized in silico to 25X coverage, and assembled using Trinity. Only the most abundant isoform for each unigene was retained for annotation and unigenes with transcripts per million mapped reads (TPM) less than 0.5 were removed from the dataset. The remaining unigenes were annotated using Trinotate with BLASTP comparisons against the Swiss-Prot/UniProt database. In addition, Pfam-A assignments were computed using hmmer, signal peptide predictions were performed using SignalP, and transmembrane domain predictions were performed using tmHMM. Gene ontology (GO assignments) were retrieved from Trinotate using the highest scoring BLASTp matches as queries.