Primer3web version 4.0.0 allows users to pick PCR primers from a DNA sequence based on criteria including:
-
oligonucleotide melting temperature, size, GC content, and primer-dimer possibilities,
-
PCR product size,
-
positional constraints within the source (template) sequence, and
-
possibilities for ectopic priming (amplifying the wrong sequence)
-
many other constraints.
All of these criteria are user-specifiable as constraints, and some are specifiable as terms in an objective function that characterizes an optimal primer pair.
Field | Value |
---|---|
Tags | |
Parent Record | |
Modified | 2022-09-28 |
Release Date | 2019-08-15 |
Identifier | 4a131184-c0cb-4ec0-8429-b6e26c5596b9 |
Publisher | MainLab, Washington State University |
License | |
Contact Name | Main, Dorrie |
Contact Email | |
Public Access Level | Public |
Program Code | 005:040 - Department of Agriculture - National Research |
Bureau Code | 005:18 - Agricultural Research Service |