Primer3web version 4.0.0 allows users to pick PCR primers from a DNA sequence based on criteria including:
oligonucleotide melting temperature, size, GC content, and primer-dimer possibilities,
PCR product size,
positional constraints within the source (template) sequence, and
possibilities for ectopic priming (amplifying the wrong sequence)
many other constraints.
All of these criteria are user-specifiable as constraints, and some are specifiable as terms in an objective function that characterizes an optimal primer pair.
|Release Date|| |
MainLab, Washington State University
|Contact Name|| |
|Public Access Level|| |
|Program Code|| |
005:040 - Department of Agriculture - National Research
|Bureau Code|| |
005:18 - Agricultural Research Service