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CottonGen Primer3

Primer3web version 4.0.0 allows users to pick PCR primers from a DNA sequence based on criteria including:

  • oligonucleotide melting temperature, size, GC content, and primer-dimer possibilities,

  • PCR product size,

  • positional constraints within the source (template) sequence, and

  • possibilities for ectopic priming (amplifying the wrong sequence)

  • many other constraints.

All of these criteria are user-specifiable as constraints, and some are specifiable as terms in an objective function that characterizes an optimal primer pair.

FieldValue
Tags
Parent Record
Modified
2022-09-28
Release Date
2019-08-15
Identifier
4a131184-c0cb-4ec0-8429-b6e26c5596b9
Publisher
MainLab, Washington State University
License
Contact Name
Main, Dorrie
Contact Email
Public Access Level
Public
Program Code
005:040 - Department of Agriculture - National Research
Bureau Code
005:18 - Agricultural Research Service