File(s) stored somewhere else
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CottonGen Primer3
Primer3web version 4.0.0 allows users to pick PCR primers from a DNA sequence based on criteria including:
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oligonucleotide melting temperature, size, GC content, and primer-dimer possibilities,
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PCR product size,
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positional constraints within the source (template) sequence, and
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possibilities for ectopic priming (amplifying the wrong sequence)
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many other constraints.
All of these criteria are user-specifiable as constraints, and some are specifiable as terms in an objective function that characterizes an optimal primer pair.
Resources in this dataset:
Resource Title: Website Pointer for CottonGen Primer3.
File Name: Web Page, url: https://www.cottongen.org/tools/primer3
Funding
Cotton Incorporated
USDA-ARS: Crop Germplasm Research Unit, College Station, TX
Bayer CropScience
Dow Chemical Company
Monsanto Company
Washington State University
USDA-NIFA: 2009-51181-06036
USDA-NIFA: 2009-51181-05808
History
Data contact name
Main, DorrieData contact email
dorrie@wsu.eduPublisher
MainLab, Washington State UniversityIntended use
Primer3web version 4.0.0 allows users to pick PCR primers from a DNA sequence.Theme
- Not specified
ISO Topic Category
- biota
- farming
National Agricultural Library Thesaurus terms
Internet; databases; genomics; breeding; translation (genetics); applied research; cotton; infrastructure; quantitative trait loci; pathogens; germplasm conservation; pedigree; unigenes; expressed sequence tags; loci; taxonomy; germplasm; Gossypium; genetic markers; publications; genetic databases; plant breeding; plant genetics; computer software; genotype; genome; transcriptome; nucleotide sequences; DNA primers; polymerase chain reactionOMB Bureau Code
- 005:18 - Agricultural Research Service
OMB Program Code
- 005:040 - National Research
ARS National Program Number
- 301
Pending citation
- No
Public Access Level
- Public