Data from: The assembled transcriptome of the adult horn fly, Haematobia irritans

The horn fly, Haematobia irritans irritans (Linnaeus, 1758; Diptera: Muscidae), a hematophagous external parasite of cattle, causes considerable economic losses to the livestock industry worldwide. This pest is mainly controlled with insecticides; however, horn fly populations from several countries have developed resistance to many of the products available for their control. In an attempt to better understand the adult horn fly and the development of resistance in natural populations, we used an Illumina paired-end read HiSeq and GAII approach to determine the transcriptomes of untreated control adult females, untreated control adult males, permethrin-treated surviving adult males and permethrin + piperonyl butoxide-treated killed adult males from a Louisiana population of horn flies with a moderate level of pyrethroid resistance. A total of 128,769,829, 127,276,458, 67,653,920, and 64,270,124 quality-filtered Illumina reads were obtained for untreated control adult females, untreated control adult males, permethrin-treated surviving adult males and permethrin + piperonyl butoxide-treated killed adult males, respectively. The de novo assemblies using CLC Genomics Workbench 8.0.1 yielded 15,699, 11,961, 2672, 7278 contigs (≥ 200 nt) for untreated control adult females, untreated control adult males, permethrin-treated surviving adult males and permethrin + piperonyl butoxide-treated killed adult males, respectively. More than 56% of the assembled contigs of each data set had significant hits in the BlastX (UniProtKB/Swiss-Prot database) (E <0.001). The number of contigs in each data set with InterProScan, GO mapping, Enzyme codes and KEGG pathway annotations were: Untreated Control Adult Females – 10,331, 8770, 2963, 2183; Untreated control adult males – 8392, 7056, 2449, 1765; Permethrin-treated surviving adult males – 1992, 1609, 641, 495; Permethrin + PBO-treated killed adult males – 5561, 4463, 1628, 1211.

Data is with this article and also available at the National Center for Biotechnology Information (NCBI) Short Read Archive (SRA) through the direct link https://www.ncbi.nlm.nih.gov/sra/SRP131897 or through SRA accession number SRP131897. The adult horn fly transcriptome Shotgun Assembly project has been deposited at DDBJ/EMBL/GenBank under the accession GGLM00000000. The version described in this paper is the first version, GGLM01000000. The overall BioProject ID is PRJNA429442 and the BioSample accessions are SAMN08355023, SAMN08355024, SAMN08355025, and SAMN08355026.

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RNA was isolated from unfed, newly emerged adult horn flies, including...
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Field	Value
Modified	2022-09-27
Release Date	2018-07-09
Identifier	fcc7601e-c7f5-4e21-9523-f9a65152fdc3
Spatial / Geographical Coverage Area	POLYGON ((-93.608485 33.018527, -91.16577 33.002096, -91.072662 32.887081, -91.143862 32.843265, -91.154816 32.640618, -91.006939 32.514649, -90.985031 32.218894, -91.105524 31.988862, -91.341032 31.846462, -91.401278 31.621907, -91.499863 31.643815, -91.516294 31.27686, -91.636787 31.265906, -91.565587 31.068736, -91.636787 30.997536, -89.747242 30.997536, -89.845827 30.66892, -89.681519 30.449842, -89.643181 30.285534, -89.522688 30.181472, -89.818443 30.044549, -89.84035 29.945964, -89.599365 29.88024, -89.495303 30.039072, -89.287179 29.88024, -89.30361 29.754271, -89.424103 29.699501, -89.648657 29.748794, -89.621273 29.655686, -89.69795 29.513285, -89.506257 29.387316, -89.199548 29.348977, -89.09001 29.2011, -89.002379 29.179192, -89.16121 29.009407, -89.336472 29.042268, -89.484349 29.217531, -89.851304 29.310638, -89.851304 29.480424, -90.032043 29.425654, -90.021089 29.283254, -90.103244 29.151807, -90.23469 29.129899, -90.333275 29.277777, -90.563307 29.283254, -90.645461 29.129899, -90.798815 29.086084, -90.963123 29.179192, -91.09457 29.190146, -91.220539 29.436608, -91.445094 29.546147, -91.532725 29.529716, -91.620356 29.73784, -91.883249 29.710455, -91.888726 29.836425, -92.146142 29.715932, -92.113281 29.622824, -92.31045 29.535193, -92.617159 29.579009, -92.97316 29.715932, -93.2251 29.776178, -93.767317 29.726886, -93.838517 29.688547, -93.926148 29.787132, -93.690639 30.143133, -93.767317 30.334826, -93.696116 30.438888, -93.728978 30.575812, -93.630393 30.679874, -93.526331 30.93729, -93.542762 31.15089, -93.816609 31.556184, -93.822086 31.775262, -94.041164 31.994339, -94.041164 33.018527, -93.608485 33.018527))
Publisher	Data in Brief
License	Creative Commons Attribution
Contact Name	Guerrero, Felix D.
Contact Email	felix.guerrero@ars.usda.gov
Public Access Level	Public
Program Code	005:040 - Department of Agriculture - National Research
Bureau Code	005:18 - Agricultural Research Service

Extended Metadata:

Field	Value
Authors	Domingues, Luisa N. (ORCID) Guerrero, Felix D. (ORCID) Cameron, Connor T. (ORCID) Farmer, Andrew (Scopus Author ID) Bendele, Kylie G. (ORCID) Foil, Lane D. (Scopus Author ID)
Peer Reviewed	No
Intended Use	Resource for investigations of the molecular basis of insecticide resistance in the horn fly, Haematobia irritans irritans. Provides candidate protein coding regions for the development of control strategies targeting adult flies.
Funding Sources	Agricultural Research Service U.S. Department of Energy DE-AC05–06OR23100
Primary Article	Domingues, L. N., Guerrero, F. D., Cameron, C., Farmer, A., Bendele, K. G., & Foil, L. D. (2018). The assembled transcriptome of the adult horn fly, Haematobia irritans. Data in Brief, 19, 1933-1940. https://doi.org/10.1016/j.dib.2018.06.095 AgID: 7249472
Method Citation	Domingues, L. N., Guerrero, F. D., Cameron, C., Farmer, A., Bendele, K. G., & Foil, L. D. (2018). The assembled transcriptome of the adult horn fly, Haematobia irritans. Data in Brief, 19, 1933-1940. https://doi.org/10.1016/j.dib.2018.06.095 AgID: 7249472
Dataset DOI (digital object identifier)	10.1016/j.dib.2018.06.095
Product Type	Dataset
Related Content	Untreated Control Females Untreated Control Males Permethrin Treated Surviving Males Permethrin + Piperonyl Butoxide Treated Killed Males
Preferred Dataset Citation	Domingues, L. N., Guerrero, F. D., Cameron, C., Farmer, A., Bendele, K. G., & Foil, L. D. (2018). The assembled transcriptome of the adult horn fly, <em>Haematobia irritans</em>. <em>Data in Brief</em>. https://doi.org/10.1016/j.dib.2018.06.095

Field	Value
ISO Topic(s)	biota farming
State or Territory	Louisiana
National Agricultural Library Thesaurus Term	transcriptome adults Haematobia irritans Muscidae parasites cattle financial economics livestock and meat industry insecticides females males permethrin Louisiana pyrethrins data collection databases insecticide resistance genomics
Ag Data Commons Keywords	Genomics & Genetics Transcriptome Transcriptome assembly Genomics & Genetics Transcriptome Genomics & Genetics

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