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Figure 2: Relative abundance of bacterial genera in life stages and tissue samples from R. microplus as detected by bTEFAP pyrosequencing.

a) Adult female cattle tick. Mean percentages (n = 2). Values below 1% were grouped as "Other" with total value of 9.5%. "Other" group includes: Staphylococcus (0.7%), Bacillus (0.5%), Streptococcus (0.7%), Vagococcus (0.3%), Pseudobutyrivibrio (0.7%), Nocardioides (0.2%), Asteroleplasma (0.9%), Ruminococcus (0.4%), Escherichia (0.9%), Acetivibrio (0.3%), Erwinia (0.1%), Pedobacter (0.2%), Dermabacter (0.1%), Ornithinicoccus (0.2%), Oribacterium (0.7%), Alkaliflexus (0.2%), Paludibacter (0.5%), Pantoea (0.2%), Cytophaga (0.1%), Mitsuokella (0.1%), Enterobacter (0.1%), Paucisalibacillus (0.4%), Lachnobacterium (0.1%), Caldithrix (0.2%), Shigella (0.1%), Solirubrobacter (0.1%), Rhodobacter (0.1%), Desulfosporosinus (0.1%). b) Adult male cattle tick. Mean percentages (n = 2). Values below 1% were grouped as "Other" with total value of 3.8%. "Other" group includes: Coxiella (0.1%), Prevotella (0.3%), Rikenella (0.1%), Pseudomonas (0.2%), Escherichia (0.3%), Hallella (0.3%), Pantoea (0.1%), Moraxella (0.7%), Arthrobacter (0.1%), Enhydrobacter (0.1%), Mogibacterium (0.1%), Kocuria (0.5%), Enterobacter (0.1%), Exiguobacterium (0.2%), Lysinibacillus (0.1%), Belnapia (0.1%). c) Cattle tick egg. Mean percentages (n = 3). Values below 1% were grouped as "Other" with total value of 6.9%. "Other" group includes: Achromobacter (0.3%), Enterococcus (0.1%), Clostridium (0.1%), Serratia (0.7%), Ruminococcus (0.3%), Propionibacterium (0.4%), Klebsiella (0.2%), Acetivibrio (0.9%), Pedobacter (0.6%), Alkaliflexus (0.4%), Centipeda (0.5%), Pantoea (0.1%), Brevibacterium (0.2%), Rubrivivax (0.4%), Enhydrobacter (0.2%), Rhodoferax (0.3%), Sporocytophaga (0.1%), Alkanindiges (0.2%), Sphingopyxis (0.1%), Caulobacter (0.1%), Trichococcus (0.1%), Comamonas (0.1%), Anaerotruncus (0.1%), Akkermansia (0.1%), Legionella (0.1%). d) Adult female cattle tick gut. Pool of tissue from five ticks tested. Values below 1% were grouped as "Other" with total value of 0.3%. "Other" group includes: Corynebacterium (0.3%). e) Adult cattle tick ovary. Pool of tissue from five ticks tested. Values below 1% were grouped as "Other" with total value of 1.8%. "Other" group includes: Borrelia (0.9%), Cryobacterium (0.9%).