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Data from: Assessment of bacterial diversity in the cattle tick Rhipicephalus (Boophilus) microplus through tag-encoded pyrosequencing

The objective of this study was to explore the R. microplus microbiome by applying the bacterial 16S tag-encoded FLX-titanium amplicon pyrosequencing (bTEFAP) technique to characterize its bacterial diversity.

Pyrosequencing was performed on adult males and females, eggs, and gut and ovary tissues from adult females derived from samples of R. microplus collected during outbreaks in southern Texas. Raw data from bTEFAP were screened and trimmed based upon quality scores and binned into individual sample collections. Bacteria identified to the species level include Staphylococcus aureus, Staphylococcus chromogenes, Streptococcus dysgalactiae, Staphylococcus sciuri, Serratia marcescens, Corynebacterium glutamicum, and Finegoldia magna. One hundred twenty-one bacterial genera were detected in all the life stages and tissues sampled. The total number of genera identified by tick sample comprised: 53 in adult males, 61 in adult females, 11 in gut tissue, 7 in ovarian tissue, and 54 in the eggs. Notable genera detected in the cattle tick include Wolbachia, Coxiella, and Borrelia. The molecular approach applied in this study allowed us to assess the relative abundance of the microbiota associated with R. microplus.

Ticks are regarded as the most relevant vectors of disease-causing pathogens in domestic and wild animals. The cattle tick, Rhipicephalus (Boophilus) microplus, hinders livestock production in tropical and subtropical parts of the world where it is endemic. Tick microbiomes remain largely unexplored.

Dataset Info

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Andreotti, Renato
Pérez de León, Adalberto A.
Dowd, Scot E.
Guerrero, Felix D.
Bendele, Kylie G.
Scoles, Glen A.
Product Type
BMC Microbiology
Contact Name
Pérez de León, Adalberto A.
Contact Email
Public Access Level
Primary Article

Andreotti, R., de León, A. A. P., Dowd, S. E., Guerrero, F. D., Bendele, K. G., & Scoles, G. A. (2011). Assessment of bacterial diversity in the cattle tick Rhipicephalus (Boophilus) microplus through tag-encoded pyrosequencing. BMC microbiology, 11(6).

AgID: 10113/55414
Funding Source(s)
Agricultural Research Service
6205-32000-031-00 D
Program Code
005:037 - Department of Agriculture - Research and Education
Bureau Code
005:18 - Agricultural Research Service
Modified Date
Release Date
Ag Data Commons Keywords: 
  • Animals & Livestock
  • Genomics & Genetics
National Agricultural Library Thesaurus Term: 
ISO Topic(s):