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Data from: Biological control of boxwood blight by Pseudomonas protegens recovered from recycling irrigation systems

Boxwood (Buxus spp.) blight caused by Calonectria pseudonaviculata (Cps) is an emerging fungal disease in the United States. It has caused significant loss to the ornamental nursery and landscape industries. In this study, 1547 bacterial strains recovered from recycling irrigation systems were evaluated in vitro and in planta for their biological control efficacy against Cps. Three most potent strains 13A3, 14D5, and 34B6 identified all belonged to Pseudomonas protegens. Strains 13A3 and 14D5 reduced Cps mycelial growth by 95 to 100% in two sets of in vitro experiments including a 48-well plate assay and a dual culture assay. Strain 34B6 also reduced Cps growth by 93% in the 48-well plate assay and 66 to 72% in dual culture plates. In planta, strain 14D5 reduced Cps infection by more than 50%, while strains 13D3 and 34B6 by 40 to 46%. Pseudomonas protegens strains produced antifungal secondary metabolites that reduced Cps growth and infection. Other possible modes of action against boxwood blight are discussed.

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FieldValue
Modified
2022-09-27
Release Date
2019-01-30
Identifier
1a2b4fb1-d229-41f2-bfdd-c14dbf7d5a9d
Spatial / Geographical Coverage Area
MULTIPOLYGON (((-75.397659 38.013497, -75.244304 38.029928, -75.375751 37.860142, -75.512674 37.799896, -75.594828 37.569865, -75.802952 37.197433, -75.972737 37.120755, -76.027507 37.257679, -75.939876 37.564388, -75.671506 37.95325, -75.397659 38.013497)), ((-76.016553 37.95325, -75.994645 37.95325, -76.043938 37.95325, -76.016553 37.95325)), ((-78.349729 39.464886, -77.82942 39.130793, -77.719881 39.322485, -77.566527 39.306055, -77.456988 39.223901, -77.456988 39.076023, -77.248864 39.026731, -77.117418 38.933623, -77.040741 38.791222, -77.128372 38.632391, -77.248864 38.588575, -77.325542 38.446175, -77.281726 38.342113, -77.013356 38.374975, -76.964064 38.216144, -76.613539 38.15042, -76.514954 38.024451, -76.235631 37.887527, -76.3616 37.608203, -76.246584 37.389126, -76.383508 37.285064, -76.399939 37.159094, -76.273969 37.082417, -76.410893 36.961924, -76.619016 37.120755, -76.668309 37.065986, -76.48757 36.95097, -75.994645 36.923586, -75.868676 36.551154, -79.510841 36.5402, -80.294043 36.545677, -80.978661 36.562108, -81.679709 36.589492, -83.673316 36.600446, -83.136575 36.742847, -83.070852 36.852385, -82.879159 36.890724, -82.868205 36.978355, -82.720328 37.044078, -82.720328 37.120755, -82.353373 37.268633, -81.969987 37.537003, -81.986418 37.454849, -81.849494 37.285064, -81.679709 37.20291, -81.55374 37.208387, -81.362047 37.339833, -81.225123 37.235771, -80.967707 37.290541, -80.513121 37.482234, -80.474782 37.421987, -80.29952 37.509618, -80.294043 37.690357, -80.184505 37.849189, -79.998289 37.997066, -79.921611 38.177805, -79.724442 38.364021, -79.647764 38.594052, -79.477979 38.457129, -79.313671 38.413313, -79.209609 38.495467, -78.996008 38.851469, -78.870039 38.763838, -78.404499 39.169131, -78.349729 39.464886)))
Publisher
Biological Control
Spatial / Geographical Coverage Location
Eastern Virginia
Temporal Coverage
January 1, 2012 to December 31, 2013
License
Creative Commons Attribution
Contact Name
Yang, Xiao
Contact Email
yxiao9@vt.edu
Public Access Level
Public
Extended Metadata: 
FieldValue
Authors
Yang, Xiao (Scopus Author ID)
Hong, Chuanxue (Scopus Author ID)
Peer Reviewed
No
Intended Use
To screen diverse bacterial strains recovered from recycling irrigation systems against Cps in dual culture, to identify promising strains to species level by DNA sequencing, and to further evaluate them using in planta assays for their potential as BCAs of boxwood blight.
Funding Sources
Animal and Plant Health Inspection Service
14-8130-0282-CA
Animal and Plant Health Inspection Service
15-8130-0282-CA
Primary Article

Yang, X., & Hong, C. (2018). Biological control of boxwood blight by Pseudomonas protegens recovered from recycling irrigation systems. Biological Control, 124, 68-73. https://doi.org/10.1016/j.biocontrol.2018.01.014

AgID: 6365586
Product Type
Dataset
FieldValue
ISO Topic(s)
biota
farming
State or Territory
Virginia
National Agricultural Library Thesaurus Term
Buxus sempervirens
blight
Calonectria
fungi
United States
landscapes
industry
irrigation systems
biological control
Pseudomonas protegens
mycelium
in vitro studies
secondary metabolites
mechanism of action
Cylindrocladium
ornamental trees
fungal diseases of plants
plant pathogenic fungi
nursery crops
biopesticides
microbial pesticides
biological control agents
microbial toxins
fungal antagonists
toxigenic strains
emerging diseases
Ag Data Commons Keywords
  • Plants & Crops
  • Plant health
  • Plants & Crops
  • Traits
  • Plants & Crops
  • Agricultural Products
  • Plants

Suggested Citations:

(original article) Yang, X., & Hong, C. (2018). Biological control of boxwood blight by Pseudomonas protegens recovered from recycling irrigation systems. Biological Control, 124, 68-73. https://doi.org/10.1016/j.biocontrol.2018.01.014

(dataset) Yang, Xiao; Hong, Chuanxue (2019). Data from: Biological control of boxwood blight by Pseudomonas protegens recovered from recycling irrigation systems. Biological Control. https://data.nal.usda.gov/dataset/data-biological-control-boxwood-blight-pseudomonas-protegens-recovered-recycling-irrigation-systems. Accessed 2023-06-04.