The ascomycete Neofusicoccum parvum, one of the causal agents of Botryosphaeria dieback, is a destructive wood‐infecting fungus and a serious threat to grape production worldwide. The capability to colonize woody tissue, combined with the secretion of phytotoxic compounds, is thought to underlie its pathogenicity and virulence. Here, we describe the repertoire of virulence factors and their transcriptional dynamics as the fungus feeds on different substrates and colonizes the woody stem. We assembled and annotated a highly contiguous genome using single‐molecule real‐time DNA sequencing. Transcriptome profiling by RNA sequencing determined the genome‐wide patterns of expression of virulence factors both in vitro (potato dextrose agar or medium amended with grape wood as substrate) and in planta. Pairwise statistical testing of differential expression, followed by co‐expression network analysis, revealed that physically clustered genes coding for putative virulence functions were induced depending on the substrate or stage of plant infection. Co‐expressed gene clusters were significantly enriched not only in genes associated with secondary metabolism, but also in those associated with cell wall degradation, suggesting that dynamic co‐regulation of transcriptional networks contributes to multiple aspects of N. parvum virulence. In most of the co‐expressed clusters, all genes shared at least a common motif in their promoter region, indicative of co‐regulation by the same transcription factor. Co‐expression analysis also identified chromatin regulators with correlated expression with inducible clusters of virulence factors, suggesting a complex, multi‐layered regulation of the virulence repertoire of N. parvum.
- Appendix S1 Supplementary tables and figures - DownloadData
Table S1: Statistics and SRA accession numbers of PacBio and Illumina genome...
- Appendix S3 Functional annotations - Downloadxlsx
Download Excel (.xlsx) file.
- Appendix S4 Normalized RNA‐sequencing counts - Downloadxlsx
Normalized RNA‐sequencing counts in the in vitro (A) and in planta (B)...
- Appendix S5 Shared motifs showing similarity to yeast motifs - Downloadxlsx
Shared motifs showing similarity to yeast motifs (MacIsaac_v1 database) and...
Dataset InfoThese fields are compatible with DCAT, an RDF vocabulary designed to facilitate interoperability between data catalogs published on the Web.
Support the prevention and management of pathogenic fungal virulence and colonization of grapevine woody stems, and serve as a basis for further studies on how N. parvum is able to colonize woody tissues or adapt to this environment. An understanding of the functions that lead to the colonization of certain cell types/tissues, and the corresponding fungal genes activated during subsequent degradation of such host tissues, may help to understand the mechanism(s) of cultivar resistance and interactions within the trunk pathogen community.
Molecular Plant Pathology
|Public Access Level|
Massonnet, M., Morales-Cruz, A., Figueroa-Balderas, R., Lawrence, D.P., Baumgartner, K., & Cantu, D. (2018). Condition-dependent co-regulation of genomic clusters of virulence factors in the grapevine trunk pathogen Neofusicoccum parvum. Molecular Plant Pathology, 19 (1), pp. 21-34
National Institute of Food and Agriculture
|Dataset DOI (digital object identifier)|
005:037 - Department of Agriculture - Research and Education
005:20 - National Institute of Food and Agriculture
- Genomics & Genetics
- Genomics & Genetics
- Plants & Crops