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Data from: Cultivar resistance to common scab disease of potato is dependent on the pathogen species

dataset
posted on 2024-02-15, 18:03 authored by Christopher ClarkeChristopher Clarke

All data from the paper "Cultivar resistance to common scab disease of potato is dependent on the pathogen species." Three separate datasets are included:

  1. A csv file with the disease severity of three common scab pathogens across 55 different potato cultivars in a greenhouse pot assay (Figures 2-5 in the associated paper). The included R script was used with this data to perform the ANOVA for the data from the greenhouse pot assay (Table 2 in the associated paper). This script can be used in R for any similar dataset to calculate the significance and percent of total variation for any number of user-defined fixed effects.

  2. A zipped file with all of the qPCR data for the expression of the txtAB genes (Figure 6 in the associated paper).

  3. An Excel file with the HPLC data for making the thaxtomin detection standard curve and quantifying the amount of thaxtomin in the test sample.


    Resources in this dataset:

    • Resource Title: Streptomyces pot assay data.

      File Name: 18.4.2updatedfileAllDataPotAssay.csv

      Resource Description: Combined data from all Streptomyces - potato pot assays from the paper "Cultivar resistance to common scab disease of potato is dependent on the pathogen species." This csv file can be used with the example R script "DiseaseseverityEstimateScript."


    • Resource Title: Combined qPCR data..

      File Name: CombinedtxtABqPCRresults.zip

      Resource Description: Zipped file that contains all qPCR data of txtAB gene expression in all experimental conditions. Combined qPCR data from Figure 6 of the paper "Cultivar resistance to common scab disease of potato is dependent on the pathogen species."


    • Resource Title: R script for estimating disease severity.

      File Name: DiseaseSeverityEstimateScript.txt

      Resource Description: R script used in combination with the "18.4.2updatedfileAllDataPotAssay.csv" file for generating the disease severity estimates (Figures 2-4) in the paper "Combined qPCR data from Figure 6 of the paper "Cultivar resistance to common scab disease of potato is dependent on the pathogen species."


    • Resource Title: Thaxtomin standard curve and quantification - All data.

      File Name: Thaxtomin_CalCurve_log_log-Scale_12072018 (003).xlsx

      Resource Description: Excel file with two sheets. The first sheet is all of the HPLC data used for calculating the standard curve of thaxtomin using known standards. The second sheet is the quantification data for the abundance of thaxtomin across the experimental groups. Data presented as Figure 6 in the paper "Combined qPCR data from Figure 6 of the paper "Cultivar resistance to common scab disease of potato is dependent on the pathogen species."

Funding

USDA-ARS: 8042-21000-283

History

Data contact name

Clarke, Christopher

Publisher

Ag Data Commons

Theme

  • Not specified

ISO Topic Category

  • biota

National Agricultural Library Thesaurus terms

cultivars; potatoes; pathogens; data collection; disease severity; greenhouses; analysis of variance; quantitative polymerase chain reaction; genes; high performance liquid chromatography; Streptomyces; gene expression; computer software

OMB Bureau Code

  • 005:18 - Agricultural Research Service

OMB Program Code

  • 005:040 - National Research

ARS National Program Number

  • 303

Pending citation

  • No

Public Access Level

  • Public

Preferred dataset citation

Clarke, Christopher R. (2019). Data from: Cultivar resistance to common scab disease of potato is dependent on the pathogen species. Ag Data Commons. https://doi.org/10.15482/USDA.ADC/1503703

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