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Data from: Dataset of de novo assembly and functional annotation of the transcriptome of blueberry (Vaccinium spp.)

dataset
posted on 2023-12-18, 18:11 authored by Xinpeng Qi, Elizabeth L. Ogden, Mark K. Ehlenfeldt, Lisa J. Rowland

Blueberry is an economically important berry crop. Both production and consumption of blueberries have increased sharply worldwide in recent years at least partly due to their known health benefits. The development of improved genomic resources for blueberry, such as a well-assembled genome and transcriptome, could accelerate breeding through genomic-assisted approaches. To enrich available transcriptome data and identify genes potentially involved in fruit quality, RNA sequencing was performed on fruit tissue from two northern-adapted hybrid blueberry breeding populations. RNA-seq was carried out using the Illumina HiSeqTM 2500 platform. Because of the absence of a reference-grade genome for blueberry, a transcriptome was de novo assembled from this RNA-seq data and other publicly available transcriptome data from blueberry downloaded from the National Center for Biotechnology Information (NCBI) Short Read Archive (SRA) using Trinity. After removing redundancy, this resulted in a dataset of 91,861 blueberry unigenes. This unigene dataset was functionally annotated using the NCBI-Nr protein database. All raw reads from the breeding populations were deposited in the NCBI SRA with accession numbers SRR6281886, SRR6281887, SRR6281888, and SRR6281889. The de novo transcriptome assembly was deposited at NCBI Transcriptome Shotgun Assembly (TSA) database with accession number GGAB00000000. These data will provide real expression evidence for the blueberry genome gene prediction and gene functional annotation and a reference transcriptome for future gene expression studies involving blueberry fruit.


Resources in this dataset:

Funding

USDA-ARS: 8042-21000-279-00

History

Data contact name

Rowland, Lisa J.

Data contact email

jeannine.rowland@usda.gov

Publisher

Data in Brief

Intended use

These transcriptome data will provide real expression evidence for the blueberry genome gene prediction and gene functional annotation and a reference transcriptome for future gene expression studies involving blueberry fruit.

Temporal Extent Start Date

2019-08-01

Theme

  • Not specified

Geographic Coverage

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ISO Topic Category

  • biota
  • farming

National Agricultural Library Thesaurus terms

blueberries; genomics; transcriptome; breeding; fruit quality; sequence analysis; hybrids; National Center for Biotechnology Information; data collection; unigenes; databases; prediction; gene expression; Vaccinium; RNA

OMB Bureau Code

  • 005:18 - Agricultural Research Service

OMB Program Code

  • 005:040 - National Research

ARS National Program Number

  • 301

Primary article PubAg Handle

Pending citation

  • No

Public Access Level

  • Public

Preferred dataset citation

Qi, Xinpeng; Ogden, Elizabeth L.; Ehlenfeldt, Mark K.; Rowland, Lisa J. (2019). Data from: Dataset of de novo assembly and functional annotation of the transcriptome of blueberry (Vaccinium spp.). Data in Brief. https://doi.org/10.1016/j.dib.2019.104390

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