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Data from: Dataset of proinflammatory cytokine and cytokine receptor gene expression in rainbow trout (Oncorhynchus mykiss) measured using a novel GeXP multiplex, RT-PCR assay

A GeXP multiplex, RT-PCR assay was developed and optimized that simultaneously measures expression of a suite of immune-relevant genes in rainbow trout (Oncorhynchus mykiss), concentrating on tumor necrosis factor and interleukin-1 ligand/receptor systems and acute phase response genes. The dataset includes expression values for drpt, il11a, il1b1, il1b2, il1b3, il1r-like-1(e3-5), il1r-like-1(e9-11), il1r1-like-a, il1r1-like-b, il1r2, saa, tnfa1, tnfa2, tnfa3, tnfrsf1a, tnfrsf1a-like-a, tnfrsf1a-like-b, tnfrsf5, and tnfrsf9. Gene expression was measured at four time-points post-challenge in both a resistant line (ARS-Fp-R) and a susceptible line (ARS-Fp-S) of rainbow trout. In addition, fish body weight, spleen index and the Flavobacterium psychrophilum load are reported. These data are an extension of information presented and discussed in the article "Proinflammatory cytokine and cytokine receptor gene expression kinetics following challenge with Flavobacterium psychrophilum in resistant and susceptible lines of rainbow trout (Oncorhynchus mykiss)" (Kutyrev et al., 2016).

Dataset Info

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FieldValue
Authors
Kutyrev, Ivan A.
(ORCID)
Cleveland, Beth M.
(ORCID)
Leeds, Timothy D.
(ORCID)
Wiens, Gregory D.
(ORCID)
Product Type
Dataset
Equipment or Software Used
Intended Use
Data describe the optimization and validation of a new GeXP assay for measuring rainbow trout proinflammatory cytokines. The data include PCR product relative migration and identification that are of use to researchers when performing this assay. The gene expression values from the rainbow trout resistant line, ARS-Fp-R, and the susceptible line, ARS-Fp-S, can be used in future studies for meta-analyses comparing the response to other pathogens or environmental conditions.
Publisher
Data in Brief
Contact Name
Wiens, Gregory D.
Contact Email
Public Access Level
Public
Preferred Dataset Citation
Kutyrev, I., Cleveland, B., Leeds, T., & Wiens, G. D. (2017). Dataset of proinflammatory cytokine and cytokine receptor gene expression in rainbow trout (<em>Oncorhynchus mykiss</em>) measured using a novel GeXP multiplex, RT-PCR assay. <em>Data in Brief</em>, 11:192-196. https://doi.org/10.1016/j.dib.2017.02.014
Primary Article

Kutyrev, I., Cleveland, B., Leeds, T., & Wiens, G. D. (2017). Dataset of proinflammatory cytokine and cytokine receptor gene expression in rainbow trout (Oncorhynchus mykiss) measured using a novel GeXP multiplex, RT-PCR assay. Data in Brief, 11:192-196.

Related Article

Kutyrev, I., Cleveland, B., Leeds, T., & Wiens, G. D. (2016). Proinflammatory cytokine and cytokine receptor gene expression kinetics following challenge with Flavobacterium psychrophilum in resistant and susceptible lines of rainbow trout (Oncorhynchus mykiss). Fish & Shellfish Immunology, 58:542-553.

Methods Citation

Kutyrev, I., Cleveland, B., Leeds, T., & Wiens, G. D. (2017). Dataset of proinflammatory cytokine and cytokine receptor gene expression in rainbow trout (Oncorhynchus mykiss) measured using a novel GeXP multiplex, RT-PCR assay. Data in Brief, 11:192-196.

License
Funding Source(s)
Agricultural Research Service
1930-32000-006
National Institute of Food and Agriculture
2012-67015-30217
Dataset DOI (digital object identifier)
10.1016/j.dib.2017.02.014
Program Code
005:037 - Department of Agriculture - Research and Education
Bureau Code
005:18 - Agricultural Research Service
Modified Date
2018-12-18
Release Date
2018-01-31
Ag Data Commons Keywords: 
  • Genomics & Genetics
  • Expression analysis
  • Genomics & Genetics
  • Animals & Livestock
  • Traits
  • Animals & Livestock
ISO Topic(s):