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Data from: Dataset of proinflammatory cytokine and cytokine receptor gene expression in rainbow trout (Oncorhynchus mykiss) measured using a novel GeXP multiplex, RT-PCR assay

A GeXP multiplex, RT-PCR assay was developed and optimized that simultaneously measures expression of a suite of immune-relevant genes in rainbow trout (Oncorhynchus mykiss), concentrating on tumor necrosis factor and interleukin-1 ligand/receptor systems and acute phase response genes. The dataset includes expression values for drpt, il11a, il1b1, il1b2, il1b3, il1r-like-1(e3-5), il1r-like-1(e9-11), il1r1-like-a, il1r1-like-b, il1r2, saa, tnfa1, tnfa2, tnfa3, tnfrsf1a, tnfrsf1a-like-a, tnfrsf1a-like-b, tnfrsf5, and tnfrsf9. Gene expression was measured at four time-points post-challenge in both a resistant line (ARS-Fp-R) and a susceptible line (ARS-Fp-S) of rainbow trout. In addition, fish body weight, spleen index and the Flavobacterium psychrophilum load are reported. These data are an extension of information presented and discussed in the article "Proinflammatory cytokine and cytokine receptor gene expression kinetics following challenge with Flavobacterium psychrophilum in resistant and susceptible lines of rainbow trout (Oncorhynchus mykiss)" (Kutyrev et al., 2016).

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FieldValue
Tags
proinflammatory cytokine
cytokine receptor gene expression
GeXP multiplex RT-PCR assay
Modified
2023-06-23
Release Date
2018-02-01
Identifier
2af96fce-1e07-4e73-84ea-b60f3d9d6b15
Publisher
Data in Brief
License
Creative Commons Attribution
Contact Name
Wiens, Gregory D.
Contact Email
greg.wiens@ars.usda.gov
Public Access Level
Public
Program Code
005:040 - Department of Agriculture - National Research
Bureau Code
005:18 - Agricultural Research Service
Extended Metadata: 
FieldValue
Authors
Kutyrev, Ivan A. (ORCID)
Cleveland, Beth M. (ORCID)
Leeds, Timothy D. (ORCID)
Wiens, Gregory D. (ORCID)
Peer Reviewed
No
Intended Use
Data describe the optimization and validation of a new GeXP assay for measuring rainbow trout proinflammatory cytokines. The data include PCR product relative migration and identification that are of use to researchers when performing this assay. The gene expression values from the rainbow trout resistant line, ARS-Fp-R, and the susceptible line, ARS-Fp-S, can be used in future studies for meta-analyses comparing the response to other pathogens or environmental conditions.
Equipment or Software Used
GeXP multiplex, RT-PCR assay
qPCR
Funding Sources
Agricultural Research Service
1930-32000-006
National Institute of Food and Agriculture
2012-67015-30217
Primary Article

Kutyrev, I., Cleveland, B., Leeds, T., & Wiens, G. D. (2017). Dataset of proinflammatory cytokine and cytokine receptor gene expression in rainbow trout (Oncorhynchus mykiss) measured using a novel GeXP multiplex, RT-PCR assay. Data in Brief, 11, 192-196. https://doi.org/10.1016/j.dib.2017.02.014

AgID: 5642527
Related Articles

Kutyrev, I., Cleveland, B., Leeds, T., & Wiens, G. D. (2016). Proinflammatory cytokine and cytokine receptor gene expression kinetics following challenge with Flavobacterium psychrophilum in resistant and susceptible lines of rainbow trout (Oncorhynchus mykiss). Fish &Amp; Shellfish Immunology, 58, 542-553. https://doi.org/10.1016/j.fsi.2016.09.053

AgID: 6118276
Method Citation

Kutyrev, I., Cleveland, B., Leeds, T., & Wiens, G. D. (2017). Dataset of proinflammatory cytokine and cytokine receptor gene expression in rainbow trout (Oncorhynchus mykiss) measured using a novel GeXP multiplex, RT-PCR assay. Data in Brief, 11, 192-196. https://doi.org/10.1016/j.dib.2017.02.014

AgID: 5642527
Dataset DOI (digital object identifier)
10.1016/j.dib.2017.02.014
Product Type
Dataset
Preferred Dataset Citation
Kutyrev, I., Cleveland, B., Leeds, T., & Wiens, G. D. (2017). Dataset of proinflammatory cytokine and cytokine receptor gene expression in rainbow trout (<em>Oncorhynchus mykiss</em>) measured using a novel GeXP multiplex, RT-PCR assay. <em>Data in Brief</em>, 11:192-196. https://doi.org/10.1016/j.dib.2017.02.014
FieldValue
ISO Topic(s)
biota
National Agricultural Library Thesaurus Term
data collection
cytokine receptors
gene expression
Oncorhynchus mykiss
reverse transcriptase polymerase chain reaction
genes
tumor necrosis factors
interleukin-1
ligands
fish
body weight
spleen
Flavobacterium psychrophilum
disease resistance
bacterial cold-water disease
immunogenetics
polymerase chain reaction
meta-analysis
pathogens
environmental factors
aquaculture
genetic lines
microbial load
quantitative polymerase chain reaction
principal component analysis
gene expression regulation
immune response
selection methods
receptors
Ag Data Commons Keywords
  • Genomics & Genetics
  • Expression analysis
  • Genomics & Genetics
  • Animals & Livestock
  • Traits
  • Animals & Livestock

Suggested Citations:

(original article) Kutyrev, I., Cleveland, B., Leeds, T., & Wiens, G. D. (2017). Dataset of proinflammatory cytokine and cytokine receptor gene expression in rainbow trout (Oncorhynchus mykiss) measured using a novel GeXP multiplex, RT-PCR assay. Data in Brief, 11, 192-196. https://doi.org/10.1016/j.dib.2017.02.014

(dataset) Kutyrev, I., Cleveland, B., Leeds, T., & Wiens, G. D (2017). Dataset of proinflammatory cytokine and cytokine receptor gene expression in rainbow trout (<em>Oncorhynchus mykiss</em>) measured using a novel GeXP multiplex, RT-PCR assay. <em>Data in Brief</em>, 11:192-196. https://doi.org/10.1016/j.dib.2017.02.014