A PCR assay targeting Borrelia spp. flagellin (flaB, 650 bp; (Barbour et al. 1996)) was used to detect presence of this bacterium in DNAs isolated from field-collected samples.
*Barbour, A. G., G. O. Maupin, G. J. Teltow, C. J. Carter, and J. Piesman. 1996. Identification of an uncultivable Borrelia species in the hard tick Amblyomma americanum: possible agent of a Lyme disease-like illness. J Infect Dis 173: 403-409
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