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Data from: Development and Validation of KASP Markers for Wheat Streak Mosaic Virus Resistance Gene Wsm2

Wheat streak mosaic virus (WSMV) can cause significant yield loss in wheat (Triticum aestivum L.) in the Great Plains of North America. A recently identified WSMV resistance gene, Wsm2, was mapped to chromosome 3BS in germplasm line 'CO960293–2'. Effective genetic markers tightly linked to the gene will enhance the selection of WSMV-resistant lines through marker-assisted selection. We have mapped Wsm2 using a high-density map developed from the wheat 90K Infinium iSelect single-nucleotide polymorphism (SNP) array with recombinant inbred lines from the cross between CO960293–2 and susceptible cultivar 'TAM 111'. Array-based SNPs that mapped within 4 cM of Wsm2 on chromosome 3BS were converted to Kompetitive Allele Specific Polymerase Chain Reaction (KASP) assays in this study. Six KASP SNPs were validated in two doubled haploid populations developed from crosses of 'RonL' × 'Ripper' and 'Snowmass' × 'Antero'. RonL and Snowmass possess the Wsm2 gene from CO960293–2. Three closely linked KASP SNPs, converted from IAAV6442, BS00018764_51, and wsnp_Ra_c16264_24873670, showed high sensitivity and specificity (0.83 ≤ sensitivity ≤ 0.97, 0.89 ≤ specificity ≤ 0.99). The latter two were also validated in six F2 breeding populations. These three KASP SNPs were effective in differentiating resistant and susceptible genotypes. Comparative mapping was performed using sequences of SNPs flanking Wsm2 and identified candidate genes and regions in Brachypodium and rice (Oryza sativa L. ssp. japonica). The KASP SNPs developed in this study should be useful for marker-assisted selection of Wsm2 in wheat breeding programs, and the newly constructed map will also facilitate map based cloning of Wsm2.

Dataset Info

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FieldValue
Authors
Tan, Chor-Tee
(ORCID)
Assanga, Silvano
(ORCID)
Zhang, Guorong
Rudd, Jackie C.
Haley, Scott D.
(ORCID)
Xue, Qingwu
(ORCID)
Ibrahim, Amir
Bai, Guihua
(ORCID)
Zhang, Xinzhong
Byrne, Patrick
Fuentealba, Maria P.
(ORCID)
Liu, Shuyu
(ORCID)
Product Type
Genome/Genetics Data
Intended Use
The KASP SNPs developed in this study should be useful for marker-assisted selection of Wsm2 in wheat breeding programs, and the newly constructed map will also facilitate map based cloning of Wsm2.
Publisher
Ag Data Commons
Contact Name
Liu, Shuyu
Contact Email
Public Access Level
Public
Preferred Dataset Citation
Tan, C., Assanga, S., Zhang, G., Rudd, J. C., Haley, S. D., Xue, Q., Ibrahim, A., Bai, G., Zhang, X., Byrne, P., Fuentealba, M. P., & Liu, S. (2017). Development and Validation of KASP Markers for Wheat Streak Mosaic Virus Resistance Gene <em>Wsm2</em>. <em>Crop Sci.</em> 57:340-349. https://doi.org/10.2135/cropsci2016.04.0234
Primary Article

Tan, C., Assanga, S., Zhang, G., Rudd, J. C., Haley, S. D., Xue, Q., Ibrahim, A., Bai, G., Zhang, X., Byrne, P., Fuentealba, M. P., & Liu, S. (2017). Development and Validation of KASP Markers for Wheat Streak Mosaic Virus Resistance Gene Wsm2. Crop Sci. 57:340-349. doi:10.2135/cropsci2016.04.0234

License
Funding Source(s)
National Institute of Food and Agriculture
2011-68002-30029
Dataset DOI (digital object identifier)
10.2135/cropsci2016.04.0234
Program Code
005:037 - Department of Agriculture - Research and Education
Bureau Code
005:18 - Agricultural Research Service
Modified Date
2018-12-17
Release Date
2018-09-07
Ag Data Commons Keywords: 
  • Genomics & Genetics
  • Genome
  • Genomics & Genetics
  • Plants & Crops
  • Traits
  • Plants & Crops
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