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Data from: Discovery of MicroRNAs of the Stable Fly (Diptera: Muscidae) by High-Throughput Sequencing

This dataset reports discovery and initial comparative analysis of 88 presumptive microRNA (miRNA) sequences from the stable fly, obtained using high-throughput sequencing of small RNAs. The majority of stable fly miRNAs were 22-23 nucleotides (nt) in length. Many miRNAs were arthropod specific, and several mature miRNA sequences showed greater sequence identity to miRNAs from other blood-feeding dipterans such as mosquitoes rather than to Drosophilids. This initial step in characterizing the stable fly microRNAome provides a basis for further analyses of life stage-specific and tissue-specific expression to elucidate their functional roles in stable fly biology.

The stable fly, Stomoxys calcitrans (L.), is a serious ectoparasite affecting animal production and health of both animals and humans. Stable fly control relies largely on chemical insecticides; however, the development of insecticide resistance as well as environmental considerations requires continued discovery research to develop novel control technologies. MicroRNAs are a class of short noncoding RNAs that have been shown to be important regulators of gene expression across a wide variety of organisms, and may provide an innovative approach with regard to development of safer more targeted control technologies.

Dataset Info

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FieldValue
Authors
Tuckow, Alexander P.
Temeyer, Kevin B.
(ORCID)
Olafson, Pia Untalan
(ORCID)
Pérez de León, Adalberto A.
(USDA)
Product Type
Genome/Genetics Data
Spatial / Geographical Coverage Location
Knipling-Bushland U.S. Livestock Insects Research Laboratory, Kerrville, TX 780280-09184
Publisher
Journal of Medical Entomology
Contact Name
Temeyer, Kevin B.
Contact Email
Public Access Level
Public
Primary Article

Tuckow, A. P., Temeyer, K. B., Olafson, P. U., & De León, A. A. P. (2013). Discovery of microRNAs of the stable fly (Diptera: Muscidae) by high-throughput sequencing. Journal of medical entomology, 50(4), 925-930.

License
Funding Source(s)
Agricultural Research Service
Program Code
005:037 - Department of Agriculture - Research and Education
Bureau Code
005:18 - Agricultural Research Service
Modified Date
2018-12-19
Release Date
2015-09-08
Ag Data Commons Keywords: 
  • Animals & Livestock
  • Animals & Livestock
  • Animal health
  • Animals & Livestock
  • Animal production
  • Genomics & Genetics
State or Territory: 
ISO Topic(s):