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Data from: Discovery of MicroRNAs of the Stable Fly (Diptera: Muscidae) by High-Throughput Sequencing

dataset
posted on 2024-02-08, 19:34 authored by Alexander P. Tuckow, Kevin B. Temeyer, Pia Untalan Olafson, Adalberto A. Pérez de León

This dataset reports discovery and initial comparative analysis of 88 presumptive microRNA (miRNA) sequences from the stable fly, obtained using high-throughput sequencing of small RNAs. The majority of stable fly miRNAs were 22-23 nucleotides (nt) in length. Many miRNAs were arthropod specific, and several mature miRNA sequences showed greater sequence identity to miRNAs from other blood-feeding dipterans such as mosquitoes rather than to Drosophilids. This initial step in characterizing the stable fly microRNAome provides a basis for further analyses of life stage-specific and tissue-specific expression to elucidate their functional roles in stable fly biology.

The stable fly, Stomoxys calcitrans (L.), is a serious ectoparasite affecting animal production and health of both animals and humans. Stable fly control relies largely on chemical insecticides; however, the development of insecticide resistance as well as environmental considerations requires continued discovery research to develop novel control technologies. MicroRNAs are a class of short noncoding RNAs that have been shown to be important regulators of gene expression across a wide variety of organisms, and may provide an innovative approach with regard to development of safer more targeted control technologies.


Resources in this dataset:

  • Resource Title: Table 1. miRNAs identified in pooled life stages of the stable fly S. calcitrans and corresponding miRBase 18 entries.

    File Name: Web Page, url: https://academic.oup.com/view-large/91020692

    miRNAs identified in pooled life stages of the stable fly S. calcitran. and corresponding miRBase 18 entries (≥10 reads, sorted by abundance).

Funding

USDA-ARS

History

Data contact name

Temeyer, Kevin B.

Data contact email

kevin.temeyer@usda.gov

Publisher

Journal of Medical Entomology

Theme

  • Not specified

Geographic location - description

Knipling-Bushland U.S. Livestock Insects Research Laboratory, Kerrville, TX 780280-09184

ISO Topic Category

  • biota
  • farming

Ag Data Commons Group

  • Veterinary Pest Genomics Center

OMB Bureau Code

  • 005:18 - Agricultural Research Service

OMB Program Code

  • 005:040 - National Research

ARS National Program Number

  • 104

Pending citation

  • No

Public Access Level

  • Public

Preferred dataset citation

Tuckow, Alexander P.; Temeyer, Kevin B.; Olafson, Pia Untalan; Pérez de León, Adalberto A. (2015). Data from: Discovery of MicroRNAs of the Stable Fly (Diptera: Muscidae) by High-Throughput Sequencing. Journal of Medical Entomology.