Animals were incorporated into pools in different proportions to estimate error and evaluate factors influencing error. Animals were incorporated into 2 types of pools, sub-pools and super pools. Within phenotype, liver abscess or normal, 16 animals were combined into 4 sub-pools, 4 animals per sub-pool in parts of 1:2:3:4. Sub-pools were constructed based on crushed frozen liver tissue mass. Within phenotype, 4 sub-pools were incorporated into 2 super pools in parts of 1:2:3:4 for super pool 1 and 3:4:1:2 for super pool 2. Super pools were made based on DNA quantity. Errors in DNA quantification would create error in forming super pools from sub-pools and variation in cell content or DNA content of liver tissue would result in error in combining sub-pools from animals.
Animal contributions to sub-pools for livers with abscess
sub-pool 1A was 1:2:3:4 parts of 15A, 36A, 35A, and 23A.
sub-pool 2A was 1:2:3:4 parts of 42A, 37A, 12A, and 22A.
sub-pool 3A was 1:2:3:4 parts of 17A, 1A, 49A, and 48A .
sub-pool 4A was 1:2:3:4 parts of 3A, 20A, 16A, and 13A.
Each part was 0.1 g of pulverized frozen liver tissue.
Animal contributions to livers without abscess
sub-pool 1N was 1:2:3:4 parts of 46N, 23N, 17N, and 12N.
sub-pool 2N was 1:2:3:4 parts of 1N, 31N, 6N, and 48N.
sub-pool 3N was 1:2:3:4 parts of 36N, 43N, 32N, and 13N.
sub-pool 4N was 1:2:3:4 parts of 34N, 19N, 41N, and 50N.
Sub-pool contributions to super pools for livers with abscess
super pool 1A was:1:2:3:4 parts sub-pool 1A, sub-pool 2A, sub-pool 3A, and sub-pool 4A.
super pool 2A was 3:4:1:2 parts sub-pool 1A, sub-pool 2A, sub-pool 3A, and sub-pool 4A.
Sub-pool contributions to super pools for livers with without abscess
super pool 1N was:1:2:3:4 parts sub-pool 1N, sub-pool 2N, sub-pool 3N, and sub-pool 4N.
super pool 2N was 3:4:1:2 parts sub-pool 1N, sub-pool 2N, sub-pool 3N, and sub-pool 4N.
Funded by the USDA Agricultural Research Service, Developing a Systems Biology Approach to Enhance Efficiency and Sustainability of Beef and Lamb Production/ 3040-31000-100-000-D
- xy data for individual animalsData
X (red) and Y (green) intensity data for 32 animals. There are 64 columns,...MD5:
- x and y data for poolsData
X (red) and Y (green) intensity for 12 pools. There are 2 columns per pool...MD5:
- Genotypes, Number of copies of B allele for BovineHD 770KData
Values are 0, 1 and 2 for 32 animals and 777,962 SNP, DNA was extracted from...MD5:
|Release Date|| |
|Spatial / Geographical Coverage Area|| |
POLYGON ((-105.0732421875 30.406044269969, -105.0732421875 39.330048552942, -92.4169921875 39.330048552942, -92.4169921875 30.406044269969))
Ag Data Commons
|Spatial / Geographical Coverage Location|| |
|Temporal Coverage|| |
April 1, 2018
|Contact Name|| |
|Public Access Level|| |
|Program Code|| |
005:040 - Department of Agriculture - National Research
|Bureau Code|| |
005:18 - Agricultural Research Service