Parasitoids in nine species of Aphelinus were collected from four aphid species on six plant species in six countries. The parasitoids were shipped or hand-carried as mummified aphids to the containment facility at the USDA-ARS, Beneficial Insect Introductions Research Unit, Newark, Delaware, and maintained under the permits indicated in the dataset. Vouchers for these populations are maintained at -20°C in 100% molecular-grade ethanol at the Beneficial Insect Introduction Research Unit, Newark, Delaware.