Spreadsheet data summarizing the collection of Solenopsis invicta female alates. Alates were collected over the period 5 June 2019 to 10 October 19 from locations across north Florida. The city, date, brief street location description, number of alates collected (Queen), latitude and longitude coordinates, designation of each alate collected, and her corresponding weight in milligrams are provided.

Photographs of agarose gel separation to detect Solenopsis invicta virus-3 in collected Solenopsis invicta queens as determined by RT-PCR. cDNA synthesized with oligonucleotide primer p812 and PCR was conducted subsequently with oligonucleotide primers p812 and p813.