Photograph of an agarose gel separation to detect Solenopsis invicta virus-3 in Solenopsis invicta queens as determined by RT-PCR. cDNA synthesized with oligonucleotide primer p812 and PCR was conducted subsequently with oligonucleotide primers p812 and p813. Lane assignments include lanes 2- 6 which represent queens collected from Pensacola, Florida, and lanes 7-11 collected from Gainesville, Florida. Lane 12 is a non-template blank and lane 1 is 1 kb DNA ladder.