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Data from: Fine mapping of the soybean aphid-resistance genes Rag6 and Rag3c from Glycine soja 85-32

Rag6 and Rag3c were delimited to a 49-kb interval on chromosome 8 and a 150-kb interval on chromosome 16, respectively. Structural variants in the exons of candidate genes were identified.

The soybean aphid, an invasive species, has significantly threatened soybean production in North America since 2000. Host-plant resistance is known as an ideal management strategy for aphids. Two novel aphid-resistance loci, Rag6 and Rag3c, from Glycine soja 85-32, were previously detected in a 10.5-cM interval on chromosome 8 and a 7.5-cM interval on chromosome 16, respectively. Defining the exact genomic position of these two genes is critical for improving the effectiveness of marker-assisted selection for aphid resistance and for identification of the functional genes. To pinpoint the locations of Rag6 and Rag3c, four populations segregating for Rag6 and Rag3c were used to fine map these two genes. The availability of the Illumina Infinium SoySNP50K/8K iSelect BeadChip, combined with single-nucleotide polymorphism (SNP) markers discovered through the whole-genome re-sequencing of E12901, facilitated the fine mapping process. Rag6 was refined to a 49-kb interval on chromosome 8 with four candidate genes, including three clustered nucleotide-binding site leucine-rich repeat (NBS–LRR) genes and an amine oxidase encoding gene. Rag3c was refined to a 150-kb interval on chromosome 16 with 11 candidate genes, two of which are a LRR gene and a lipase gene. Moreover, by sequencing the whole-genome exome-capture of the resistant source (E12901), structural variants were identified in the exons of the candidate genes of Rag6 and Rag3c. The closely linked SNP markers and the candidate gene information presented in this study will be significant resources for integrating Rag6 and Rag3c into elite cultivars and for future functional genetics studies.

Dataset Info

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FieldValue
Authors
Zhang, Shichen
Zhang, Zhongnan
Wen, Zixiang
Gu, Cuihua
An, Yong-Qiang Charles
Bales, Carmille
DiFonzo, Christina
Song, Qijian
(ORCID)
Wang, Dechun
Product Type
Dataset
Publisher
Springer
Contact Name
Wang, Dechun
Contact Email
Public Access Level
Public
Primary Article

Zhang, S., Zhang, Z., Wen, Z., Gu, C., An, Y., & Bales, C. et al. (2017). Fine mapping of the soybean aphid-resistance genes Rag6 and Rag3c from Glycine soja 85-32. Theoretical And Applied Genetics, 130(12), 2601-2615.

License
Funding Source(s)
National Institute of Food and Agriculture
Modified Date
2018-12-04
Release Date
2018-09-07
Ag Data Commons Keywords: 
  • Genomics & Genetics
  • Genome
  • Genomics & Genetics
ISO Topic(s):