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Data from: First Report of Squash vein yellowing virus in Watermelon in Guatemala

Watermelon (Citrullus lanatus) and other cucurbits are important crops grown in Guatemala for local consumption and export. The whitefly (Bemisia tabaci) vector of Cucurbit yellow stunting disorder virus (CYSDV), Melon chlorotic leaf curl virus (MCLCuV), and Squash vein yellowing virus (SqVYV) was observed in fields with numbers increasing during the season. Four samplings of crowns, peduncles, and/or leaves of symptomatic plants were made in March and April 2015. Total RNA was extracted from symptomatic plant tissue and tested by RT-PCR for SqVYV, CYSDV, Papaya ringspot virus (PRSV), and/or begomoviruses. Primers specific for the coat protein gene of SqVYV (1020 bp), CYSDV (707 bp), or PRSV (511 bp), and degenerate begomovirus primers (1159 or 533 bp) amplified products of the expected sizes from 15 of 24, 20 of 24, 4 of 24, or 8 of 8 plants, respectively. SqVYV amplicons from six individual plants from the fourth sampling, SqVYV and CYSDV amplicons from a pool of plants from the third sampling, and degenerate begomovirus amplicons from the second sampling were cloned in the pGEM-T vector. Five clones of each amplicon were sequenced in both directions and representative consensus sequences were deposited in GenBank (Accession Nos. KT007178 to KT007183). Sequence analysis demonstrated that SqVYV coat protein gene sequences from Guatemala shared 99 to 100% nucleotide (nt) identity with each other, and 97 to 98% nt identity with divergent SqVYV isolates previously described from Florida (e.g., WM2005aHi, GenBank Accession No. JF897974) and California (GenBank Accession No. KP218061), but only 90% nt identity with the predominant SqVYV isolate found in Florida (e.g., Sq2003Hi, GenBank Accession No. EU259611) . Tissue blots were prepared from crowns and peduncles from the first, third, and fourth samplings, and tested by tissue blot nucleic acid hybridization assay for SqVYV. Tissue blots indicated SqVYV infection in an additional 48 of 102 watermelon samples, and cylindrical inclusions typical of SqVYV were observed in phloem tissue from the fourth sampling by light microscopy, confirming the identification of SqVYV. This is the first report of SqVYV infecting watermelon in Central America.

Dataset Info

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FieldValue
Authors
Jeyaprakash, Ayyamperumal
Baker, Carlye A.
Schubert, Timothy S.
Badillo-Vargas, Ismael E.
(ORCID)
Roberts, Pamela D.
(ORCID)
Funderburk, Joseph E.
Adkins, Scott
(ORCID)
Product Type
Genome/Genetics Data
Spatial / Geographical Coverage Area
POLYGON ((-90.095555 13.735338, -90.608624 13.909771, -91.23241 13.927832, -91.689747 14.126218, -92.22775 14.538829, -92.20323 14.830103, -92.087216 15.064585, -92.229249 15.251447, -91.74796 16.066565, -90.464473 16.069562, -90.438867 16.41011, -90.600847 16.470778, -90.711822 16.687483, -91.08167 16.918477, -91.453921 17.252177, -91.002269 17.254658, -91.00152 17.817595, -90.067934 17.819326, -89.14308 17.808319, -89.150806 17.015577, -89.229122 15.886938, -88.930613 15.887273, -88.604586 15.70638, -88.518364 15.855389, -88.225023 15.727722, -88.68068 15.346247, -89.154811 15.066419, -89.22522 14.874286, -89.145535 14.678019, -89.353326 14.424133, -89.587343 14.362586, -89.534219 14.244816, -89.721934 14.134228, -90.064678 13.88197, -90.095555 13.735338))
Spatial / Geographical Coverage Location
Guatemala
Temporal Coverage
2015-03/2015-04
Publisher
Plant Health Progress
Contact Name
Adkins, Scott
Contact Email
Public Access Level
Public
Primary Article

Jeyaprakash, A., Baker, C. A., Schubert, T. S., Badillo-Vargas, I. E., Roberts, P. D., Funderburk, J. E. & Adkins, S. (2015). First Report of Squash vein yellowing virus in Watermelon in Guatemala. Plant Health Progress, 16(3) 113-114

License
Dataset DOI (digital object identifier)
10.1094/PHP-BR-15-0019
Program Code
005:037 - Department of Agriculture - Research and Education
Bureau Code
005:18 - Agricultural Research Service
Modified Date
2018-12-10
Release Date
2018-09-21
Ag Data Commons Keywords: 
  • Plants & Crops
  • Plant health
  • Plants & Crops
  • Genomics & Genetics
  • Expression analysis
  • RNA Seq
  • Genomics & Genetics
  • Expression analysis
  • Genomics & Genetics
  • Sequence Assembly
  • Genomics & Genetics
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