This study produced a comprehensive transcriptome from newly molted adult ticks and will provide a useful resource for studies of tick feeding and host perception and also assist genome annotation refinements.
The goal was to develop a comprehensive transcriptome of adult female Rhipicephalus microplus ticks and discover transcriptome changes that are related to feeding and host-perception prior to feeding. We have sequenced the transcriptomes of unfed newly molted adult female ticks, newly molted adult females that were feeding upon a bovine host for 3 hours following attachment to the host, and newly molted adult females that were placed in a gas-permeable bag attached to a bovine host for 3 hours (attachment to host prevented but host odors available to the ticks). There were 2 biological replicates and transcriptomes assembled from each individual experimental condition. A collective adult female transcriptome was assembled from sequence data pooled from all 3 conditions (using data from all replicates). Differential gene expression was examined using all possible comparisons to detect putative feeding-associated and host perception-associated transcripts.
Field | Value |
---|---|
Tags | |
Modified | 2022-09-28 |
Release Date | 2019-10-08 |
Identifier | a7ca0914-7ab4-4555-b20b-25cb6d5d9b62 |
Spatial / Geographical Coverage Area | POINT (-98.32857 26.38684) |
Publisher | National Center for Biotechnology Information |
Spatial / Geographical Coverage Location | USDA-ARS Cattle Fever Tick Research Laboratory, United States Department of Agriculture, 22675 N. Moorefield Rd, Edinburg, TX 78541, USA |
Temporal Coverage | November 17, 2008 to November 18, 2008 |
License | |
Contact Name | Bendele, Kylie |
Contact Email | |
Public Access Level | Public |
Program Code | 005:040 - Department of Agriculture - National Research |
Bureau Code | 005:18 - Agricultural Research Service |