U.S. flag

An official website of the United States government

Other Access

The information on this page (the dataset metadata) is also available in these formats:

JSON RDF

via the DKAN API

Data Extent

Data from: Gene expression during the early stages of host perception and attachment in adult female Rhipicephalus microplus ticks

This study produced a comprehensive transcriptome from newly molted adult ticks and will provide a useful resource for studies of tick feeding and host perception and also assist genome annotation refinements.

The goal was to develop a comprehensive transcriptome of adult female Rhipicephalus microplus ticks and discover transcriptome changes that are related to feeding and host-perception prior to feeding. We have sequenced the transcriptomes of unfed newly molted adult female ticks, newly molted adult females that were feeding upon a bovine host for 3 hours following attachment to the host, and newly molted adult females that were placed in a gas-permeable bag attached to a bovine host for 3 hours (attachment to host prevented but host odors available to the ticks). There were 2 biological replicates and transcriptomes assembled from each individual experimental condition. A collective adult female transcriptome was assembled from sequence data pooled from all 3 conditions (using data from all replicates). Differential gene expression was examined using all possible comparisons to detect putative feeding-associated and host perception-associated transcripts.

FieldValue
Tags
Modified
2022-03-03
Release Date
2019-10-08
Identifier
a7ca0914-7ab4-4555-b20b-25cb6d5d9b62
Spatial / Geographical Coverage Area
POINT (-98.32857 26.38684)
Publisher
National Center for Biotechnology Information
Spatial / Geographical Coverage Location
USDA-ARS Cattle Fever Tick Research Laboratory, United States Department of Agriculture, 22675 N. Moorefield Rd, Edinburg, TX 78541, USA
Temporal Coverage
November 17, 2008 to November 18, 2008
License
Contact Name
Bendele, Kylie
Contact Email
Public Access Level
Public
Program Code
005:040 - Department of Agriculture - National Research
Bureau Code
005:18 - Agricultural Research Service