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Other Access

The information on this page (the dataset metadata) is also available in these formats:


via the DKAN API

Data Extent

Data From: Habitat type and host grazing regimen influence the soil microbial diversity and communities within potential biting midge larval habitats

Culicoides biting midges are important vectors of diverse microbes such as viruses, protozoa, and nematodes that cause diseases in wild and domestic animals. However, little is known about the role of microbial communities in midge larval habitat utilization in the wild. In this study, we characterized microbial communities (bacterial, protistan, fungal and metazoan) in soils from disturbed (bison and cattle grazed) and undisturbed (non-grazed) pond and spring potential midge larval habitats. We evaluated the influence of habitat and grazing disturbance and their interaction on microbial communities, diversity, presence of midges, and soil properties. These data can be used to better understand environmental microbial communities in tallgrass prairie ecosystems associated with grazed versus ungrazed pond and spring habitats and to draw inferences on the interactions of these communities and soil properties with the presence of biting midge larvae. These data should not be used to make inferences for ecosystems other than tallgrass prairie, for animal management methods other than open cow-calf or bison grazing (such as feedlots, dairies, or stockyards), or for other grazing mammals (such as sheep or goats). These data were collected between the months of September and December and therefore are not representative of microbial communities present from January through August. Abbreviations used include Total Carbon (TC), Total Nitrogen (TN), Organic Matter (OM), Konza Prairie Biological Station (KPBS), Operational Taxonomic Unit (OTU), Principal Coordinates Analysis (PCoA), ribosomal RNA (rRNA), and vesicular stomatitis virus (VSV).

The raw Illumina MiSeq sequence data for this project can be found here: https://www.ncbi.nlm.nih.gov/bioproject/PRJNA862140

Resources in this dataset:

  • Resource Title: Metadata for Midge Larval Habitat Soil Microbiome
    File Name: Metadata for NCBI Accession PRJNA862140.xlsx
    Resource Description: This spreadsheet links the raw sequence reads on NCBI with data on the presence/absence of Culicoides midges and soil chemistry data (% total soil nitrogen, % total soil carbon, and % organic matter).
Release Date
Spatial / Geographical Coverage Area
POLYGON ((-96.609477996826 39.109633808188, -96.608791351318 39.073127836213, -96.537380218506 39.07392743942, -96.539783477783 39.126414882492, -96.539783477783 39.136534867789, -96.548023223877 39.13600227324, -96.548366546631 39.115760695362, -96.567249298096 39.115760695362, -96.567249298096 39.112031349217, -96.575145721436 39.113096896827, -96.575489044189 39.109101010216, -96.586818695068 39.109633808188, -96.584758758545 39.11549432004, -96.590938568115 39.11362966459, -96.597805023193 39.113896046961, -96.597461700439 39.108568208217))
Ag Data Commons
Spatial / Geographical Coverage Location
Konza Prairie Biological Station, Located south of Manhattan, KS
Temporal Coverage
September 4, 2020 to December 10, 2020
Contact Name
McGregor, Bethany
Contact Email
Public Access Level
Program Code
005:040 - Department of Agriculture - National Research
Bureau Code
005:18 - Agricultural Research Service