Insect sex pheromones (SPs) are central to mate‐finding behaviour, and play an essential role in the survival and reproduction of organisms. Understanding the roles, biosynthetic pathways and evolution of insect chemical communication systems has been an exciting challenge for biologists. Compared with Lepidoptera, little is known about the mechanisms underlying pheromone biosynthesis in Hemiptera. In this study, we isolated and characterized two new desaturase‐like genes, termed Asutdes1 and Asutdes2, from Adelphocoris suturalis, an important agricultural pest in China. Although the two genes encode an identical protein, Southern blot analysis revealed that they are duplicated genes. The Asutdes2 transcript is more abundant than Asutdes1 in the tissues tested, in particular the metathoracic scent gland and fat body. Silencing Asutdes expression in females by injecting double‐stranded RNA (dsAsutdes) against a portion of the coding sequence shared by the two genes enhanced the production of (E)‐4‐oxo‐2‐hexenal, a component of the A. suturalis SP blend, and dramatically suppressed the sexual attractiveness of A. suturalis females. We conclude that dsAsutdes is associated with the SP biosynthetic pathway in A. suturalis.
- Table S1. List of species names and corresponding published accession numbers for sequences used in the desaturase phylogeny - Download docxData
Gene abbreviation, Species Name, and Accession number.
Dataset InfoThese fields are compatible with DCAT, an RDF vocabulary designed to facilitate interoperability between data catalogs published on the Web.
To isolate and characterize two new desaturase‐like genes from Adelphocoris suturalis, an important agricultural pest in China, and contribute to the undersdanding of mechanisms underlying pheromone biosynthesis in Hemiptera.
Insect Molecular Biology
|Public Access Level|
Luo, J. , Li, Z. , Ma, C. , Zhang, Z. , Hull, J. J., Lei, C. , Jin, S. & Chen, L. (2017), Knockdown of a metathoracic scent gland desaturase enhances the production of (E)‐4‐oxo‐2‐hexenal and suppresses female sexual attractiveness in the plant bug Adelphocoris suturalis. Insect Molecular Biology, 26: 642-653
Ministry of Agriculture of the People's Republic of China
|Dataset DOI (digital object identifier)|
- Genomics & Genetics
- Agroecosystems & Environment
- Agricultural pests