Data from: Net release and uptake of xenometabolites across intestinal, hepatic, muscle, and renal tissue beds in healthy conscious pigs

Data and supplemental figures for manuscript titled "Net release and uptake of xenometabolites across intestinal, hepatic, muscle, and renal tissue beds in healthy conscious pigs." We used a catheterized conscious pig model to estimate inter-organ flux of xenometabolites, derivatives, and bile acids using LC/MS. Female pigs (n=12; 2-3 months old; 25.6 ± 2.2 kg) had surgically-implanted catheters across portal-drained viscera (PDV), splanchnic area (SPL), liver, kidney, and hindquarter muscle. Overnight fasted arterial and venous plasma was collected simultaneously in a conscious state and stored at -80°C. Thawed samples were analyzed by liquid chromatography-mass spectrometry. Plasma flow was determined with para-aminohippuric acid dilution technology and used to calculate net organ balance for each metabolite. This repository contains the LC/MS data and supplemental figures for the submitted manuscript.

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Organ Net Balance Data for Mercer et al.xlsx
Calculated net balances for LC/MS data from Mercer et al.

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LC/MS data for Mercer et al.xlsx
LC/MS peak area data for xenometabolites in Mercer et al.

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Mercer et al Supplemental Figure 2pdf
Supplemental Figure 2 for Mercer et al manuscript: Absolute differences of...
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Mercer et al Supplemental Figure 1pdf
Supplemental Figure 1 for Mercer et al manuscript: Supplemental Figure 1:...
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Supplemental Table 1 for Mercer et al.xlsx
Mercer et al Supplemental Table 1: Raw differences of identified...
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Field	Value
Tags	metabolomics xenometabolite pig model tissue flux inter organ balance NP107
Modified	2022-09-28
Release Date	2020-04-29
Identifier	a2950fe2-e4db-4151-b39a-c490943d17ed
Publisher	Ag Data Commons
License	U.S. Public Domain
Contact Name	Piccolo, Brian
Contact Email	bdpiccolo@uams.edu
Public Access Level	Public
Program Code	005:040 - Department of Agriculture - National Research
Bureau Code	005:18 - Agricultural Research Service

Extended Metadata:

Field	Value
Authors	Mercer, Kelly E. (Scopus Author ID) Ten Have, Gabriella A. M. (ORCID) Pack, Lindsay (Scopus Author ID) Lan, Renny (ORCID) Deutz, Nicolaas E. P. (ORCID) Adams, Sean H. (ORCID) Piccolo, Brian D. (ORCID)
Peer Reviewed	No
Intended Use	LC/MS data may be used to estimate inter-organ flux of identified metabolites. The catheterized pig model system is a unique in vivo assessment of organ uptake and release. These data confirm gastrointestinal origin for several microbial derived xenometabolites and suggest that liver and kidneys are uptake sites.
Use Limitations	Data are observational and do not prove gastrointestinal absorption and do not provide mechanistic information. Only portal, splanchnic, liver, kidney and muscle organs can be assessed in this model, so data cannot rule out release/uptake from other organs. Identified metabolites are based on in-house standard library at the Arkansas Children's Nutrition Center and is not a comprehensive profile of all microbial derived xenometabolites.
Funding Sources	Agricultural Research Service 6026-51000-012-06S
Primary Article	Mercer, K. E., Ten Have, G. A. M., Pack, L., Lan, R., Deutz, N. E. P., Adams, S. H., & Piccolo, B. D. (2020). Net release and uptake of xenometabolites across intestinal, hepatic, muscle, and renal tissue beds in healthy conscious pigs. American Journal of Physiology-Gastrointestinal and Liver Physiology, 319(2), G133-G141. https://doi.org/10.1152/ajpgi.00153.2020 AgID: 7111150
Method Citation	Mercer, K. E., Ten Have, G. A. M., Pack, L., Lan, R., Deutz, N. E. P., Adams, S. H., & Piccolo, B. D. (2020). Net release and uptake of xenometabolites across intestinal, hepatic, muscle, and renal tissue beds in healthy conscious pigs. American Journal of Physiology-Gastrointestinal and Liver Physiology, 319(2), G133-G141. https://doi.org/10.1152/ajpgi.00153.2020 AgID: 7111150
Dataset DOI (digital object identifier)	10.15482/USDA.ADC/1518604
Product Type	Dataset

Field	Value
OSTI Identifier
ISO Topic(s)	biota health
National Agricultural Library Thesaurus Term	intestines muscles swine animal models bile acids females catheters liver kidneys frozen storage storage temperature liquid chromatography mass spectrometry metabolites
Ag Data Commons Keywords	Food & Nutrition Nutrition Food & Nutrition

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