The longhorned tick, Haemaphysalis longicornis, feeds upon a wide range of bird and mammalian hosts. Mammalian hosts include cattle, deer, sheep, goats, humans, and horses. This tick is known to transmit a number of pathogens causing tick-borne diseases, and was the vector of a recent serious outbreak of oriental theileriosis in New Zealand. A New Zealand-USA consortium was established to sequence, assemble, and annotate the genome of this tick, using ticks obtained from New Zealand's North Island. In New Zealand, the tick is considered exclusively parthenogenetic and this trait was deemed useful for genome assembly. Very high molecular weight genomic DNA was sequenced on the Illumina HiSeq4000 and the long-read Pac Bio Sequel platforms. Twenty-eight SMRT cells produced a total of 21.3 million reads which were assembled with Canu on a reserved supercomputer node with access to 12 TB of RAM, running continuously for over 24 days. The final assembly dataset consisted of 34,211 contigs with an average contig length of 215,205 bp. The quality of the annotated genome was assessed by BUSCO analysis, an approach that provides quantitative measures for the quality of an assembled genome. Over 95% of the BUSCO gene set was found in the assembled genome. Only 48 of the 1066 BUSCO genes were missing and only 9 were present in a fragmented condition. The raw sequencing reads and the assembled contigs/scaffolds are archived at the National Center for Biotechnology Information.
Funded by USDA-ARS Knipling-Bushland US Livestock Insects Research Laboratory CRIS project 3094-32000-036-00
- BioProject PRJNA540490html
Haemaphysalis longicornis (longhorned tick)...
- BioSample: SAMN11539514html
New Zealand cattle tick- Massey University February 2018 Collection
- SRR9226158 Illumina HiSeq Haemaphysalis longicornishtml
SRX5996658: Illumina Genome Sequencing of the longhorned tick, Haemaphysalis...
- SRR9226159 PacBio Sequel Haemaphysalis longicornishtml
SRX5996659: PacBio Genome Sequencing of the longhorned tick, Haemaphysalis...
- Genome VFIB01000000html
Haemaphysalis longicornis isolate New Zealand Cattle Tick FotT, whole genome...
|Release Date|| |
|Spatial / Geographical Coverage Location|| |
Massey University, School of Veterinary Sciences, Palmerston North, New Zealand
National Center for Biotechnology Information
|Temporal Coverage|| |
February 4, 2018
|Contact Name|| |
|Public Access Level|| |
|Program Code|| |
005:040 - Department of Agriculture - National Research
|Bureau Code|| |
005:18 - Agricultural Research Service