Ag Data Commons
Browse

File(s) stored somewhere else

Please note: Linked content is NOT stored on Ag Data Commons and we can't guarantee its availability, quality, security or accept any liability.

Data from: The testes transcriptome of the New World Screwworm, Cochliomyia hominivorax

dataset
posted on 2024-02-09, 18:21 authored by Kylie G. Bendele, Felix D. Guerrero, Connor T. Cameron, Adalberto A. Pérez de León

The New World Screwworm (NWS), Cochliomyia hominivorax, is a pest insect that is endemic to subtropical and tropical regions of the Western Hemisphere. The female lays eggs in open wounds or orifices of warm-blooded animals. Upon hatching, the resulting larvae feed upon the host's living tissues, which can become infected and death can occur. The sterile insect technique was developed to eradicate this pest from North America and new female conditional-lethal strains that generate only male individuals are being developed for use in the eradication program. To facilitate the identification of useful transcripts and gene promoters for these new strains, an Illumina Hi-Seq protocol was used to sequence the testes transcriptome of NWS. This study reports the assembly of 4149 transcripts (≥200 nt) from testes dissected from NWS males obtained from the J06 strain used in the screwworm production plant in Pacora, Panama. Functional annotation resulted in 2060, 2031, 558, and 325 transcripts with assigned BlastX, Gene Ontology, Enzyme Codes, and KEGG pathway information, respectively. In the Gene Ontology annotations, 6% and 3% of the transcripts in the Biological Process Ontology were noted as Developmental Process and Reproduction, respectively. Supplementary data includes FastA sequences of the final assembled dataset contigs and a table of Functional annotation information for the NWS testes transcripts. This dataset will serve as a resource to facilitate studies of sex determination in the NWS and the development of recombinant vectors that can be used to create new male-only strains of NWS.


Resources in this dataset:

  • Resource Title: The testes transcriptome of the New World Screwworm, Cochliomyia hominivorax.

    File Name: Web Page, url: https://www.sciencedirect.com/science/article/pii/S2352340916306862

    Data in Brief article reporting the assembly of 4149 transcripts (≥200 nt) from testes dissected from New World Screwworm, Cochliomyia hominivoraxmales males obtained from the J06 strain used in the screwworm production plant in Pacora, Panama.

Funding

USDA-ARS: CRIS Project No. 6205–32000-031-00

USDA-APHIS: Interagency Agreement No. 34-WT-07–5000-1992-IA

History

Data contact name

Guerrero, Felix David

Data contact email

Felix.Guerrero@ars.usda.gov

Publisher

Data in Brief

Intended use

This dataset will serve as a resource to facilitate studies of sex determination in the NWS and the development of recombinant vectors that can be used to create new male-only strains of NWS. Testes-specific transcript sequences to supplement the available NWS transcriptomes previously reported for embryos, larvae, adult male, and adult female. Resource for investigations of sex-specific gene expression and sex determination pathways. Provides candidate protein coding regions and gene promoters for the development of recombinant vectors that can be used to create new male-only strains of NWS.

Theme

  • Not specified

Geographic location - description

Panama

ISO Topic Category

  • biota

Ag Data Commons Group

  • Veterinary Pest Genomics Center

National Agricultural Library Thesaurus terms

Cochliomyia hominivorax; insect pests; tropics; females; animals; death; insect larvae; hatching; tissues; sterile insect technique; North America; males; genes; standard operating procedures; testes; transcriptome; Panama; gene ontology; reproduction; data collection; sex determination; adults; gene expression; open reading frames

OMB Bureau Code

  • 005:18 - Agricultural Research Service

OMB Program Code

  • 005:040 - National Research

ARS National Program Number

  • 104

Primary article PubAg Handle

Pending citation

  • No

Public Access Level

  • Public

Preferred dataset citation

Bendele, K. G., Guerrero, F. D., Cameron, C., Perez de Leon, A. A. (2016). The testes transcriptome of the New World Screwworm, Cochliomyia hominivorax, Data in Brief, 9:1141-1146. https://doi.org/10.1016/j.dib.2016.11.026

Usage metrics

    Licence

    Exports

    RefWorks
    BibTeX
    Ref. manager
    Endnote
    DataCite
    NLM
    DC