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Data from: The testes transcriptome of the New World Screwworm, Cochliomyia hominivorax

The New World Screwworm (NWS), Cochliomyia hominivorax, is a pest insect that is endemic to subtropical and tropical regions of the Western Hemisphere. The female lays eggs in open wounds or orifices of warm-blooded animals. Upon hatching, the resulting larvae feed upon the host's living tissues, which can become infected and death can occur. The sterile insect technique was developed to eradicate this pest from North America and new female conditional-lethal strains that generate only male individuals are being developed for use in the eradication program. To facilitate the identification of useful transcripts and gene promoters for these new strains, an Illumina Hi-Seq protocol was used to sequence the testes transcriptome of NWS. This study reports the assembly of 4149 transcripts (≥200 nt) from testes dissected from NWS males obtained from the J06 strain used in the screwworm production plant in Pacora, Panama. Functional annotation resulted in 2060, 2031, 558, and 325 transcripts with assigned BlastX, Gene Ontology, Enzyme Codes, and KEGG pathway information, respectively. In the Gene Ontology annotations, 6% and 3% of the transcripts in the Biological Process Ontology were noted as Developmental Process and Reproduction, respectively. Supplementary data includes FastA sequences of the final assembled dataset contigs and a table of Functional annotation information for the NWS testes transcripts. This dataset will serve as a resource to facilitate studies of sex determination in the NWS and the development of recombinant vectors that can be used to create new male-only strains of NWS.

Dataset Info

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FieldValue
Authors
Bendele, Kylie G.
(ORCID)
Guerrero, Felix D.
(ORCID)
Cameron, Connor T.
(ORCID)
Pérez de León, Adalberto A.
(USDA)
Product Type
Dataset
Spatial / Geographical Coverage Location
Panama
Intended Use
This dataset will serve as a resource to facilitate studies of sex determination in the NWS and the development of recombinant vectors that can be used to create new male-only strains of NWS. Testes-specific transcript sequences to supplement the available NWS transcriptomes previously reported for embryos, larvae, adult male, and adult female. Resource for investigations of sex-specific gene expression and sex determination pathways. Provides candidate protein coding regions and gene promoters for the development of recombinant vectors that can be used to create new male-only strains of NWS.
Publisher
Data in Brief
Contact Name
Guerrero, Felix David
Contact Email
Public Access Level
Public
Preferred Dataset Citation
Bendele, K. G., Guerrero, F. D., Cameron, C., Perez de Leon, A. A. (2016). The testes transcriptome of the New World Screwworm, <em>Cochliomyia hominivorax</em>, <em>Data in Brief</em>, 9:1141-1146. https://doi.org/10.1016/j.dib.2016.11.026
Primary Article

Bendele, K. G., Guerrero, F. D., Cameron, C., Perez de Leon, A. A. (2016). The testes transcriptome of the New World Screwworm, Cochliomyia hominivorax, Data in Brief, 9:1141-1146

Methods Citation

Bendele, K. G., Guerrero, F. D., Cameron, C., Perez de Leon, A. A. (2016). The testes transcriptome of the New World Screwworm, Cochliomyia hominivorax, Data in Brief, 9:1141-1146

License
Funding Source(s)
Agricultural Research Service
CRIS Project No. 6205–32000-031-00
Animal and Plant Health Inspection Service
Interagency Agreement No. 34-WT-07–5000-1992-IA
Dataset DOI (digital object identifier)
10.1016/j.dib.2016.11.026
Program Code
005:037 - Department of Agriculture - Research and Education
Bureau Code
005:18 - Agricultural Research Service
Modified Date
2018-12-04
Release Date
2018-01-18
Ag Data Commons Keywords: 
  • Genomics & Genetics
  • Transcriptome
  • Transcriptome assembly
  • Genomics & Genetics
  • Transcriptome
  • Animals & Livestock
  • Genomics & Genetics
ISO Topic(s):