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ELIGULUM-A regulates lateral branch and leaf development. Original figure files

Original record with links to JPG and TIFF images, zip files with data, and pdf files

Contents list:

File View/Open Description Size Format

  • Figure legends.pdf Description of figures 1-4, 6 19.87Kb PDF
  • Supplemental Figure Legends.pdf Description of figures S2-3, S5, S7-8, S11 18.44Kb PDF
  • Fig 1A.JPG Plant adult characteristics, Bowman-cul2.b-rob1 2.236Mb JPEG image
  • Fig 1B.JPG Plant adult characteristics, Bowman-cul2.b-rob1; eli-a.17 2.305Mb JPEG image
  • Fig 1C.JPG Plant adult characteristics, Non-mutant Bowman cultivar 2.360Mb JPEG image
  • Fig 1D.JPG Plant adult characteristics, Bowman-eli-a.17 2.332Mb JPEG image
  • Fig 1E.JPG Plant adult characteristics, Bowman-eli-a.18 2.204Mb JPEG image
  • Fig 1F.JPG Plant adult characteristics, Bowman-cul2.b-rob1; eli-a.18 2.040Mb JPEG image
  • Fig 1G.JPG Plant adult characteristics, eli-a.14 2.292Mb JPEG image
  • Fig 1H.JPG Plant adult characteristics, cul2.b-rob1; eli-a.14 2.185Mb JPEG image
  • Fig 2A.tif Ligular region in eli-a alleles, Non-mutant Bowman 2.359Mb TIFF image
  • Fig 2B.tif Ligular region in eli-a alleles, Bowman-eli-a.17 3.754Mb TIFF image
  • Fig 2C.tif Ligular region in eli-a alleles; Bowman-eli-a.18 3.754Mb TIFF image
  • Fig 2D.tif Ligular region in eli-a alleles; Heterozygous Bowman-eli-a.17/eli-a.18 3.754Mb TIFF image
  • Fig 2E.TIF SEM of the ligular regions, Non-mutant Bowman 3.559Mb TIFF image
  • Fig 2F.TIF SEM of the ligular regions, Bowman-eli-a.18 3.555Mb TIFF image
  • Fig 3A.tif Non-mutant Bowman shoot apical meristem 2.737Mb TIFF image
  • Fig 3B.tif Bowman axillary bud #3 2.737Mb TIFF image
  • Fig 3C.tif Bowman axillary bud #2 2.778Mb TIFF image
  • Fig 3D.tif Bowman-cul2.b-rob1 shoot apical meristem 3.044Mb TIFF image
  • Fig 3E.tif Bowman-eli-a.17 shoot apical meristem 3.802Mb TIFF image
  • Fig 3F.tif Bowman-eli-a.17 axillary bud 3.802Mb TIFF image
  • Fig 3G.tif Bowman-eli-a.17; cul2.b-rob1 shoot apical meristem 2.884Mb TIFF image
  • Fig 3H.tif Section through axillary bud #1 seen in panel G 3.802Mb TIFF image
  • Fig 4A.JPG Comparison of leaves from mature non-mutant Bowman and Bowman-eli-a.18 plants 1.986Mb JPEG image
  • Fig 4B.tif Midrib from non-mutant plant; Safranin-O stained 12.93Mb TIFF image
  • Fig 4C.tif Midrib from Bowman-eli-a.18 plant; Safranin-O stained 12.81Mb TIFF image
  • Fig 4D.tif Leaf vein from non-mutant plant, Safranin-O stained 3.801Mb TIFF image
  • Fig 4E.tif Leaf vein from Bowman-eli-a.18 plant; Safranin-O stained 3.801Mb TIFF image
  • Fig 4F.tif Leaf margin from non-mutant plant; Safranin-O stained 3.801Mb TIFF image
  • Fig 4G.tif Leaf margin from Bowman-eli-a.18 plant; Safranin-O stained 3.801Mb TIFF image
  • Fig 6A.tif Longitudinal section of four day-old non-mutant shoot apex 3.549Mb TIFF image
  • Fig 6B.tif Transverse section of four day-old non-mutant shoot apex 1.438Mb TIFF image
  • Fig 6C.tif Close-up view of ligule circled in panel A 3.549Mb TIFF image
  • Fig 6D.tif Longitudinal section of four-day-old cul2.b shoot apex 3.549Mb TIFF image
  • Fig 6E.tif Transverse section of four-day-old cul2.b shoot apex 3.549Mb TIFF image
  • Fig 6F.tif ELI-A staining observed in same location as adaxial HvLg1 staining 5.235Mb TIFF image
  • Fig 6G.tif HvLg1 staining detected on adaxial and abaxial surfaces 6.115Mb TIFF image
  • Fig 6H.tif ELI-A expression in leaf axils and axillary buds associated with vascular bundles 3.801Mb TIFF image
  • Figure S2A.JPG Allelism test showing suppression of cul2.b by eli-a.17/eli-a.18 2.117Mb JPEG image
  • Figure S2B.JPG Phenotype of eli-a.9/eli-a9 2.818Mb JPEG image
  • Figure S2C.JPG Allelism test illustrating the plant phenotype in an eli-a.9/eli-a.18; CUL2/CUL2 plant 1.981Mb JPEG image
  • Figure S2D.JPG Suppression of cul2 by eli-a.9 2.271Mb JPEG image
  • Figure S3A1.tif Ligule and auricle development in plants carrying different eli-a alleles 2.784Mb TIFF image
  • Figure S3A2.tif Ligule and auricle development in plants carrying different eli-a alleles 3.754Mb TIFF image
  • Figure S3A3.tif Ligule and auricle development in plants carrying different eli-a alleles 3.754Mb TIFF image
  • Figure S3A4.tif Ligule and auricle development in plants carrying different eli-a alleles 3.294Mb TIFF image
  • Figure S3A5.tif Ligule and auricle development in plants carrying different eli-a alleles 3.754Mb TIFF image
  • Figure S3A6.tif Ligule and auricle development in plants carrying different eli-a alleles 3.015Mb TIFF image
  • Figure S3A7.tif Ligule and auricle development in plants carrying different eli-a alleles 3.168Mb TIFF image
  • Figure S3A8.tif Ligule and auricle development in plants carrying different eli-a alleles 3.269Mb TIFF image
  • Figure S3B.TIF Partial rudimentary ligule is ocasionally seen in eli-a.18 plants 7.880Mb TIFF image
  • Figure S5A.tif Seven-day-old shoot apex stained with Toluidine Blue, Bowman-eli-a.18 25.49Mb TIFF image
  • Figure S5B.tif Seven-day-old shoot apex stained with Toluidine Blue, Close-up of axillary bud 12.18Mb TIFF image
  • Figure S7A.JPG Mature non-mutant Bowman spike 2.144Mb JPEG image
  • Figure S7B.JPG Mature Bownman-eli-a.18 spike with compact arrangement of spikelets 2.245Mb JPEG image
  • Figure S7C.JPG Comparison of Bowman-eli-a.3, Bowman-eli-a.3; cul2.b-rob1, and Bowman-cul2.b-rob1 2.377Mb JPEG image
  • Figure S7D.JPG Comparison of Bowman-eli-a.17, Bowman-eli-a.17; cul2b-rob1, and Bowman-cul2.b-rob1 2.327Mb JPEG image
  • Figure S8A.tif Adult culms from the non-mutant cultivar Bowman 12.10Mb TIFF image
  • Figure S8B.tif Adult culms from eli-a.18 12.09Mb TIFF image
  • Figure S8C.tif Adult culms from eli-a.3 12.16Mb TIFF image
  • Figure S11A.tif ELI-A sense control on four-day-old non-mutant shoot apex 3.549Mb TIFF image
  • Figure S11B.tif Four-day-old non-mutant shoot apex hybridized with ELI-A probe 12.99Mb TIFF image
  • Figure S11C.tif ELI-A expression in a developing ligule 5.186Mb TIFF image
  • Figure S11D.tif Transverse section showing the band of ELI-A transcript continuing across the leaf blade 47.18Mb TIFF image
  • Figure S11E.tif HgLg1 sense control on four-day-old non-mutant shoot apex 12.91Mb TIFF image
  • Figure S11F.tif ELI-A transcript occasionally seen at the boundary between the developing axillary bud and leaf axil 5.207Mb TIFF image
  • Figure S11G.tif Serial transverse section from a four-day-old non-mutant shoot apex 27.31Mb TIFF image
  • Figure S11H.tif Serial transverse section from a four-day-old non-mutant shoot apex with axillary bud circled 27.35Mb TIFF image
  • Fig 1A-H.zip All figures: Mutant and non-mutant plant characteristics 17.71Mb application/zip
  • Fig 2A-F.zip All figures: Ligular region in eli-a alleles 18.32Mb application/zip
  • Fig 3A-H.zip All figures: Longitudinal sections of seven-day-old shoot apices from non-mutant and mutant lines stained with Toluidine Blue 23.04Mb application/zip
  • Fig 4A-G.zip All figures: Secondary cell wall development 21.73Mb application/zip
  • Fig 6A-H.zip All figures: ELI-A expression in non-mutant and cul2.b seedlings 23.80Mb application/zip
  • Figure S2A-S2D.zip All figures: Genetic testing of eli-a alleles 9.057Mb application/zip
  • Figure S3A-S3B.zip All figures: Ligule development in eli-a alleles 30.82Mb application/zip
  • Figure S5A-S5B.zip All figures: Axillary bud development in Bowman-eli-a.18 28.68Mb application/zip
  • Figure S7A-S7D.zip All figures: Eli-a spike phenotypes 8.980Mb application/zip
  • Figure S8A-S8C.zip All figures: Secondary cell wall development 18.24Mb application/zip
  • Figure S11A-S11H.zip All figures: ELI-A in situ hybridizations 92.07Mb application/zip