i5k Workspace

About the i5k Workspace@NAL

The i5k Workspace (https://i5k.nal.usda.gov) is an inclusive genome portal for any arthropod genome project that would like to make use of our resources. We provide download services, BLAST, the JBrowse genome browser, and the Apollo manual curation service. Over 50 arthropod genomes are now part of the i5k Workspace, and users are encouraged to browse the genomes that we host, and contribute to the curation of each genome. For more information about the i5k Workspace, you can read our paper on the i5k Workspace, view our posters and talks, and find our software projects on github. The Ag Data Commons is now hosting a growing number of i5k Workspace datasets.

About the i5k initiative

The i5k initiative is a transformative project that aims to sequence and analyze the genomes of 5,000 arthropod species. The National Agricultural Library has partnered with the i5k initiative to create the i5k Workspace@NAL, which serves any ‘orphaned’ arthropod genome project's hosting needs. For more information about the i5k initiative, read the paper and visit the website.

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i5k Datasets

45 datasets

Genes of viral origin in the Fopius arisanus genome

Fopius arisanus (Sonan) is a braconid wasp (subfamily Opiinae) and biological control agent of a broad range of tephritid fruit fly species, including the global pests Mediterranean fruit fly Ceratitis capitata and the Oriental fruit fly Bactrocera dorsalis. In an effort to create foundational genomic resources for this species, the complete genome and transcriptomes for several wasp life stages have been recently generated. Manual annotation of 55 viral genes and phylogenetic analysis revealed that F. arisanus has independently acquired a symbiotic virus related to alpha-nudiviruses.

insects 5000 program

Gerris buenoi genome annotations v0.5.3

The Gerris buenoi genome was recently sequenced and annotated as part of the i5k pilot project by the Baylor College of Medicine. This dataset presents the Gerris buenoi gene set BCM_v_0.5.3, which was generated computationally. RNA-Seq data was used with additional protein homology data for a MAKER automated annotation of the Gerris buenoi genome assembly 1.0. NOTE: This gene set is an unstable pre-release (v0.5.3), and was provided to facilitate manual curation and analyses before the official gene set is released. Gene identifiers from this gene set will likely not be maintained.

insects 5000 program

Gerris buenoi Official Gene set v1.0

The Gerris buenoi genome was recently sequenced and annotated as part of the i5k pilot project by the Baylor College of Medicine. The Gerris buenoi research community has manually reviewed and curated the computational gene predictions and generated an official gene set, OGSv1.0. The OGS is an integration of automatic gene predictions from Maker (performed by Dan Hughes at Baylor College of Medicine) with manual annotations by the research community (done via the Apollo manual annotation software).

insects 5000 program

Gerris buenoi Genome Assembly 1.0

The Baylor College of Medicine recently sequenced and annotated the Gerris buenoi genome as part of the i5k pilot project. This dataset presents the Gerris buenoi genome v1.0. This assembly version is the pre-release version, prior to filtering and quality control by the National Center for Biotechnology Information's GenBank resource. The corresponding genome assembly in GCA_001010745.1

insects 5000 program

Blattella germanica Official Gene Set OGSv1.0

The Blattella germanica genome was recently sequenced and annotated as part of the i5k pilot project by the Baylor College of Medicine. The Blattella germanica research community has manually reviewed and curated the computational gene predictions and generated an official gene set, OGSv1.0.

insects 5000 program

Cimex Lectularius Official Gene Set v1.1

The Baylor College of Medicine recently sequenced and annotated the Cimex lectularius genome as part of the i5k pilot project. The C. lectularius research community has manually reviewed and curated the computational gene predictions and generated an official gene set, OGSv1.2. This dataset presents the Cimex lectularius Official Gene Set (OGS) v1.1. The OGS is an integration of automatic gene predictions from MAKER with manual annotations by the research community.

insects 5000 program

Orussus abietinus mitochondrial genome assembly

The Baylor College of Medicine has sequenced and annotated the Orussus abietinus genome as part of the i5k pilot project. This dataset represents a targeted assembly and annotation of the mitochondrial genome.

insects 5000 program

Athalia rosae mitochondrial genome assembly

The Baylor College of Medicine has sequenced and annotated the Athalia rosae genome as part of the i5k pilot project. This dataset represents a separate targeted assembly of the mitochondrial genome.

insects 5000 program

Orussus abietinus Official Gene Set OGSv1.0

The Orussus abietinus genome was recently sequenced and annotated as part of the i5k pilot project by the Baylor College of Medicine. The Orussus abietinus research community has manually reviewed and curated the computational gene predictions and generated an official gene set, OGSv1.0. The general procedure for generating this OGS is outlined here: https://github.com/NAL-i5K/I5KNAL_OGS/wiki. OGSv1.0 was generated by merging gene set OABI-V0.5.3-Models generated by the Baylor College of Medicine, and community-curated models in the Apollo software, after QC of the Apollo output.

insects 5000 program