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i5k Workspace

About the i5k Workspace@NAL

The i5k Workspace (https://i5k.nal.usda.gov) is an inclusive genome portal for any arthropod genome project that would like to make use of our resources. We provide download services, BLAST, the JBrowse genome browser, and the Apollo manual curation service. Over 50 arthropod genomes are now part of the i5k Workspace, and users are encouraged to browse the genomes that we host, and contribute to the curation of each genome. For more information about the i5k Workspace, you can read our paper on the i5k Workspace, view our posters and talks, and find our software projects on github. The Ag Data Commons is now hosting a growing number of i5k Workspace datasets.

About the i5k initiative

The i5k initiative is a transformative project that aims to sequence and analyze the genomes of 5,000 arthropod species. The National Agricultural Library has partnered with the i5k initiative to create the i5k Workspace@NAL, which serves any ‘orphaned’ arthropod genome project's hosting needs. For more information about the i5k initiative, read the paper and visit the website.

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i5k Datasets

15 datasets

Hyalella azteca Official Gene Set v1.0

    The Hyalella azteca genome was recently sequenced and annotated as part of the i5k pilot project by the Baylor College of Medicine. The Hyalella azteca research community has manually reviewed and curated the computational gene predictions and generated an official gene set, OGSv1.0. The OGS is an integration of automatic gene predictions from Maker with manual annotations by the research community (via the Apollo manual annotation software).

    Onthophagus taurus Genome Assembly 1.0

      The Baylor College of Medicine recently sequenced and annotated the Onthophagus taurus genome as part of the i5k pilot project. This dataset presents the Onthophagus taurus genome v1.0. This assembly version is the pre-release version, prior to filtering and quality control by the National Center for Biotechnology Information's GenBank resource.

      Data from: Tripal EUtils - A Tripal module to increase exchange and reuse of genome assembly metadata

        A core component of NCBI’s BioSample metadata are the BioSample “packages” ([https://www.ncbi.nlm.nih.gov/biosample/docs/packages/](https://www.ncbi.nlm.nih.gov/biosample/docs/packages/)). Data submitters can choose a package, which contain a variety of attribute sets, such as plant- or insect-specific attributes, attribute values as recommended by the MIxS standard, etc. Here, we provide suggested ontology term mappings for attributes from the Invertebrate 1.0 and Plant 1.0 packages. This dataset corresponds to Table 4 in the corresponding publication in the journal Database.

        Chelonus insularis Official Gene Set OGSv1.0

          This Official Gene Set is an integration ([NCBI Cephus cinctus Annotation Release 101](https://www.ncbi.nlm.nih.gov/genome/annotation_euk/Cephus_cinctus/101/)) from NCBI's eukaryotic annotation pipeline v8.0 with manual annotations by the research community (done via the Apollo manual annotation software). QC and Merge of the dataset was performed using the GFF3toolkit software ([https://github.com/NAL-i5K/GFF3toolkit](https://github.com/NAL-i5K/GFF3toolkit)).

          Genes of viral origin in the Microplitis demolitor genome

            *Microplitis demolitor* (Hymenoptera: Braconidae) is a parasitoid used as a biological control agent to control larval-stage Lepidoptera and serves as a model for studying the function and evolution of symbiotic viruses in the genus Bracovirus. Using RNA-Seq data for this species and manual annotation of genes of viral origin, we annotated a high-quality gene set including 171 virus-derived protein-coding genes.

            Microplitis demolitor Official Gene Set micdem_OGSv1.0

              This dataset presents the *Microplitis demolitor* Official Gene Set (OGS) v1.0. The OGS is an integration of automatic gene predictions from *Microplitis demolitor* genome annotations NCBI-RefSeq's gene set NCBI Microplitis demolitor Annotation Release 101, with manual annotations by the research community, performed via the Apollo manual curation software. Manual annotations were QC'd via the GFF3toolkit and NCBI's table2asn_GFF software, and merged with NCBI Microplitis demolitor Annotation Release 101 via the GFF3toolkit.

              Cimex Lectularius Genome Assembly 1.0

                The Baylor College of Medicine recently sequenced and annotated the Cimex lectularius genome as part of the i5k pilot project. The C. lectularius research community has manually reviewed and curated the computational gene predictions and generated an official gene set, OGSv1.2. This dataset presents the Cimex lectularius genome v1.0. This assembly version is the pre-release version, prior to filtering and quality control by the National Center for Biotechnology Information's GenBank resource.