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i5k Workspace

About the i5k Workspace@NAL

The i5k Workspace (https://i5k.nal.usda.gov) is an inclusive genome portal for any arthropod genome project that would like to make use of our resources. We provide download services, BLAST, the JBrowse genome browser, and the Apollo manual curation service. Over 50 arthropod genomes are now part of the i5k Workspace, and users are encouraged to browse the genomes that we host, and contribute to the curation of each genome. For more information about the i5k Workspace, you can read our paper on the i5k Workspace, view our posters and talks, and find our software projects on github. The Ag Data Commons is now hosting a growing number of i5k Workspace datasets.

About the i5k initiative

The i5k initiative is a transformative project that aims to sequence and analyze the genomes of 5,000 arthropod species. The National Agricultural Library has partnered with the i5k initiative to create the i5k Workspace@NAL, which serves any ‘orphaned’ arthropod genome project's hosting needs. For more information about the i5k initiative, read the paper and visit the website.

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i5k Datasets

97 datasets

Chelonus insularis Official Gene Set OGSv1.0

    This Official Gene Set is an integration ([NCBI Cephus cinctus Annotation Release 101](https://www.ncbi.nlm.nih.gov/genome/annotation_euk/Cephus_cinctus/101/)) from NCBI's eukaryotic annotation pipeline v8.0 with manual annotations by the research community (done via the Apollo manual annotation software). QC and Merge of the dataset was performed using the GFF3toolkit software ([https://github.com/NAL-i5K/GFF3toolkit](https://github.com/NAL-i5K/GFF3toolkit)).

    Genes of viral origin in the Fopius arisanus genome

      *Fopius arisanus* (Sonan) is a braconid wasp (subfamily Opiinae) and biological control agent of a broad range of tephritid fruit fly species, including the global pests Mediterranean fruit fly *Ceratitis capitata* and the Oriental fruit fly *Bactrocera dorsalis*. In an effort to create foundational genomic resources for this species, the complete genome and transcriptomes for several wasp life stages have been recently generated. Manual annotation of 55 viral genes and phylogenetic analysis revealed that *F. arisanus* has independently acquired a symbiotic virus related to alpha-nudiviruses.

      Genes of viral origin in the Microplitis demolitor genome

        *Microplitis demolitor* (Hymenoptera: Braconidae) is a parasitoid used as a biological control agent to control larval-stage Lepidoptera and serves as a model for studying the function and evolution of symbiotic viruses in the genus Bracovirus. Using RNA-Seq data for this species and manual annotation of genes of viral origin, we annotated a high-quality gene set including 171 virus-derived protein-coding genes.

        Drosophila biarmipes genome annotations v0.5.3 for genome assembly Dbia_2.0

          RNA-Seq data were used with additional protein homology data for a MAKER automated annotation of the Drosophila biarmipes genome assembly Dbia 2.0

          NOTE: This gene set is an unstable pre-release (v0.5.3), and is provided to facilitate manual curation and analyses before the official gene set is released. Gene identifiers from this gene set will likely not be maintained.

          Oncopeltus fasciatus genome annotations v0.5.3

            The Oncopeltus fasciatus genome was recently sequenced and annotated as part of the i5k pilot project by the Baylor College of Medicine. The O. fasciatus research community has manually reviewed and curated the computational gene predictions and generated an official gene set, OGSv1.1. This dataset presents the Oncopeltus fasciatus gene set BCM_v_0.5.3, which was generated computationally. RNA-Seq data was used with additional protein homology data for a MAKER automated annotation of the Oncopeltus fasciatus genome assembly 1.0.