U.S. flag

An official website of the United States government

Updated Bottom Up Solution for Triacylglycerols

    This Microsoft Excel workbook (2.712 Mb) contains spreadsheets that implement the Updated Bottom Up Solution for Triacylglycerols that allows Critical Ratios that provide structural information from mass spectrometry to be used to reconstitute the original mass spectra from which they came. It contains spreadsheets that use the non-nested equations (Critical Limits explicitly shown), as well as spreadsheets that used the nested equations (Case determinations made with Critical Limits not explicitly shown).

    Quantification of TAGs by LC-MS

      This Microsoft Excel workbook contains spreadsheets for calculating response-factor normalized triacylglycerol (TAG) compositions from response factors derived from GC-FID of fatty acid methyl esters (FAME). This workbook (1.160 Mb) reads the output from a ThermoScientific XCalibur Quan Browser •Excel Short Report• (In XCalibur: File • Export data to Excel • Export Short Excel report), and requires input of the FA composition determined by GC-FID (manually entered or read from Excel spreadsheet [provided upon request]).

      Isotope-corrected Critical Ratio calculations

        This Microsoft Excel workbook (168.259 Mb) calculates Critical Ratios for triacylglycerols based on manually entered mass spectra, with isotope correction by isotope patterns calculated at www.ChemCalc.org. Average mass spectra across integrated peaks are manually entered according to the instructions in the Supplementary Material to the publication 'The Updated Bottom Up Solution for Mass Spectrometry of Soybean Oil in a Dietary Supplement Gelcap".

        Data from: Assessment of town and park characteristics related to physical activity in the Lower Mississippi Delta

          The Delta Neighborhood Physical Activity Study was an observational study designed to assess characteristics of neighborhood built environments associated with physical activity. It was an ancillary study to the Delta Healthy Sprouts Project and therefore included towns and neighborhoods in which Delta Healthy Sprouts participants resided. The 12 towns were located in the Lower Mississippi Delta region of Mississippi. Data were collected via electronic surveys between August 2016 and September 2017 using the Rural Active Living Assessment (RALA) tools and the Community Park Audit Tool (CPAT). Scale scores for the RALA Programs and Policies Assessment and the Town-Wide Assessment were computed using the scoring algorithms provided for these tools via SAS software programming.

          Data from: Chondroitin sulfate disaccharides modified the structure and function of the murine gut microbiome under healthy and stressed conditions

            Chondroitin sulfate (CS) has been widely used for medical and nutraceutical purposes due to its roles in maintaining tissue structural integrity. We investigated if CS disaccharides may act as a bioactive compound and modulate gut microbial composition in mice. Our data show that CS disaccharides supplementation for 16 days significantly reduced blood LPS in the mice experiencing exhaustive exercise stress. CS disaccharides partially restored total fecal short-chain fatty acids from the level significantly repressed in mice under the stress. Our findings demonstrated that CS was likely butyrogenic and resulted in a significant increase in fecal butyrate concentration. CS disaccharides had a profound impact on gut microbial composition, affecting the abundance of 13.6% and 7.3% Operational Taxonomic Units in fecal microbial communities in healthy and stressed mice, respectively. CS disaccharides reduced the prevalence of inflammatory Proteobacteria. Together, our findings demonstrated that CS may ameliorate stress-induced intestinal inflammation. Furthermore, CS significantly increased intestinal *Bacteroides acidifaciens* population, indirectly exerting its immunomodulatory effect on the intestine. CS disaccharides had a significant impact on a broad range of biological pathways under stressed condition, such as ABC transporters, two-component systems, and carbohydrate metabolism. Our results will facilitate the development of CS as a bioactive nutraceutical.

            NEWT: National Extension Web-mapping Tool

              National Extension Web-mapping Tool (or NEWT), is the key in efforts to make spatial data available within the Cooperative Extension System. NEWT requires no GIS experience to use. NEWT provides access to relevant spatial data at a variety of scales (national, state, county) in useful formats (maps, tables, graphs),