Uniform Soybean Tests, Northern Region

The Uniform Soybean Tests, Northern Region, in place since 1941, evaluate yield, disease resistance, and quality traits of public breeding lines from northern states of the USA and Canadian provinces. The annual reports which compile the test results (PDF format) are available, and new reports are added annually.

Genomics and Genetics

De novo transcriptome assembly and annotations for wheat curl mite (Aceria tosichella)

To study the impact of wheat streak mosaic virus on global gene expression in wheat curl mite, we generated a de novo transcriptome assembly using 50 x 50 paired end reads from the Illumina HiSeq 2500. Reads were assembled using Trinity (version 2.0.6) and contigs greater than 200 nt were retained. All assembled transcripts were annotated using the Trinotate pipeline using blastp searches against the Swiss-prot/Uni-Prot database, blastx searches against the Swiss-prot/Uni-Prot databases, HMM searches against the Pfam-A database, blastp searches against the non-redundant protein database, and signalP and tmHMM predictions. To reduce noise from low abundance transcripts not well supported by the data, we filtered the assembly to retain only those transcripts with TPM values >=0.5.

Genomics and Genetics

Annotations of Unigenes Assembled from Schizaphis graminum and Sipha flava

Transcriptomes were assembled de novo from pools of adult aphids that were feeding on sorghum and switchgrass. Reads from all replicates were pooled, normalized in silico to 25X coverage, and assembled using Trinity. Only the most abundant isoform for each unigene was retained for annotation and unigenes with transcripts per million mapped reads (TPM) less than 0.5 were removed from the dataset. The remaining unigenes were annotated using Trinotate with BLASTP comparisons against the Swiss-Prot/UniProt database. In addition, Pfam-A assignments were computed using hmmer, signal peptide predictions were performed using SignalP, and transmembrane domain predictions were performed using tmHMM. Gene ontology (GO assignments) were retrieved from Trinotate using the highest scoring BLASTp matches as queries.

Genomics and Genetics

Rocky Mountain Region (Region 2) Geospatial Data

Our geographic area of responsibility covers Colorado, Kansas, Nebraska, South Dakota, and Wyoming. The datasets presented here are derived from the USFS Land Status Records System (LSRS) and the USFS infrastructure database (Infra), and other current projects of a region wide nature, then processed using ArcMap and Google™ Earth Pro.

Maps and Multimedia

NRCS Regional Conservation Partnership Program - Prairie Grasslands Region

One of the most threatened ecosystems in North America, native prairie and grasslands contained within the Prairie Grasslands Region are essential habitat for a number of wild game and threatened species, including the lesser prairie chicken and sage grouse. The region also encompasses the Red River Basin of the North and the Ogallala Aquifer—areas that are facing critical conservation needs on working lands from frequent flooding and ponding (in the north) to prolonged drought and aquifer decline (in the Ogallala). This dataset includes a printer-friendly CCA map and shapefiles for GIS.

Maps and Multimedia

Long-Term Agricultural Research (LTAR) network - Meteorological Collection

The LTAR network maintains stations for standard meteorological measurements including, generally, air temperature and humidity, shortwave (solar) irradiance, longwave (thermal) radiation, wind speed and direction, barometric pressure, and precipitation. Many sites also have extensive comparable legacy datasets. The LTAR scientific community decided that these needed…

Agroecosystems & Environment