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Data from: Deer keds and blacklegged ticks infesting ungulates in the United States: molecular detection of Bartonella spp., Rickettsia spp., Anaplasma spp., and Borrelia spp.

    Deer keds are blood-feeding flies from which several human and animal pathogens have been detected, including the causative agent of Lyme Disease (Borrelia burgdorferi). Cervids, which are the primary hosts of deer keds, are not natural reservoirs of B. burgdorferi, and it has been suggested that deer keds may acquire bacterial pathogens by co-feeding near ticks that are infected with the bacteria. We tested this hypothesis by using a molecular assay to screen for presence of Anaplasma spp., Bartonella spp., Borrelia spp., and Rickettsia spp. in specimens of European deer keds (n=306) and blacklegged ticks (n=315) collected from 38 individual white-tailed deer in Pennsylvania. There was limited similarity in the bacterial DNA detected between these ectoparasites per host, suggesting that co-feeding may not be a mechanism by which deer keds acquire these bacteria.

    2014 Swine CAFO Study SE for Agricultural Antibiotic Resistance in Mississippi State, Mississippi

      The purpose of this study was to measure the microbial constituents of swine-sow, -nursery, and -finisher farm manure lagoon wastewater and determine the changes induced by farm management. A total of 37 farms were visited in the Mid-South USA and analyzed for the genes 16S rRNA, spaQ (Salmonella spp.), Camp-16S (Campylobacter spp.), tetA, tetB, ermF, ermA, mecA, and intI using quantitative PCR. Additionally, 16S rRNA sequence libraries were created.

      Data from: Retention of Pantoea agglomerans Sc1R across stadia of the southern green stink bug, Nezara viridula (L.) (Hemiptera: Pentatomidae)

        This study aims to determine the acquisition of the bacterium Pantoea agglomerans strain Sc1R by early instars of Southern green stink bug, Nezara viridula, and determine persistence of P. agglomerans Sc1R across subsequent stadia. 1st and 2nd instars were exposed to P. agglomerans Sc1R and subsequently maintained to adulthood; cohorts were sampled at 3rd and 5th instars, as well as adults.

        Nebraska Prairie Study for Agricultural Antibiotic Resistance in Lincoln, Nebraska

          The inherent spatial heterogeneity and complexity of antibiotic resistant bacteria and antibiotic resistance (AR) genes in manureaffected soils makes it difficult to sort out resistance that can be attributed to human antibiotic use from resistance that occurs naturally in the soil. This study characterizes native Nebraska prairie soils that have not been affected by human or food-animal waste products to provide data on background levels of resistance in southeastern Nebraskan soils.

          Data from: A database for global soil health assessment

            The SoilHealthDB database integrates soil health measurements conducted in the field from sites across the globe. SoilHealthDC currently focuses on four main conservation management methods: cover crops, no-tillage, agro-forestry systems, and organic farming. These studies represent 354 geographic sites (i.e., locations with unique latitudes and longitudes) in 42 countries around the world. The SoilHealthDB includes 42 soil health indicators and 46 background indicators that describe factors such as climate, elevation, and soil type.

            Data from: Comparative analysis of genome sequences from four strains of the Buchnera aphidicola Mp endosymbiont of the green peach aphid, Myzus persicae

              Myzus persicae, the green peach aphid, is a polyphagous herbivore that feeds on hundreds of species of mostly dicot crop plants. Like other phloem-feeding aphids, M. persicae rely on the endosymbiotic bacterium, Buchnera aphidicola (Buchnera Mp), for biosynthesis of essential amino acids and other nutrients that are not sufficiently abundant in their phloem sap diet. To determine whether the endosymbiotic bacteria of M. persicae could play a role in tobacco adaptation, the Buchnera Mp genomes from two tobacco-adapted and two non-tobacco M. persicae lineages are sequenced.