The eDNA samples in the eDNAtlas database describe species occurrence locations and were collected by the U.S. Forest Service and numerous agencies that have partnered with the National Genomics Center for Wildlife and Fish Conservation (NGC) throughout the United States. The eDNAtlas is accessed via an interactive ArcGIS Online (AGOL) map that allows users to view and download sample site information and lab results of species occurrence for the U.S. The results are primarily based on samples analyzed at the National Genomics Center for Wildlife and Fish Conservation (NGC) and associated with geospatial attributes created by the Boise Spatial Streams Group (BSSG).
The bull trout (Salvelinus confluentus) eDNA survey results Online Map allows users to view the survey results in an interactive map by coupling 1) predictions from the range-wide, spatially precise Climate Shield model on the location of natal habitats of bull trout with 2) a sampling template for every 8-digit hydrologic unit in the historical range of bull trout, based on the probability of detecting bull trout presence using environmental DNA (eDNA) sampling. The map provides the ability to zoom in and look at an area of interest, as well as to create queries or select an area to download points as a shapefile.
The milkweed bug, Oncopeltus fasciatus, was sequenced as part of the i5k pilot project from Baylor College of Medicine (Illumina data). To augment those resources, we present here a hybrid genome assembly with low coverage PacBio data, assembled with PBJelly: the Oncopeltus fasciatus Hybrid Genome Assembly v1.0.
Data from: Draft genome sequences of eight streptogramin-resistant Enterococcus species isolates from animal and environmental sources in the United States
Draft genome sequences of five Enterococcus faecium, two Enterococcus hirae, and one Enterococcus gallinarum from enviromental sources and chicken carcass rinsates. Isolates were selected for their resistance to the streptogramin antibiotic, Quinupristin-Dalfopristin and were all collected in the United States between 2001 and 2004. Antimicrobial resistance genes were identified conferring resistance to the macrolide-lincosamide-streptogramins, aminoglycosides, tetracycline, beta-lactams, and glycopeptides.
Data from: Identification of Single-Nucleotide Polymorphic Loci Associated with Biomass Yield under Water Deficit in Alfalfa (Medicago sativa L.) Using Genome-Wide Sequencing and Association Mapping
Alfalfa is a worldwide grown forage crop and is important due to its high biomass production and nutritional value. However, the production of alfalfa is challenged by adverse environmental factors such as drought and other stresses. Developing drought resistance alfalfa is an important breeding target for enhancing alfalfa productivity in arid and semi-arid regions. In the present study, we used genotyping-by-sequencing and genome-wide association to identify marker loci associated with biomass yield under drought in the field in a panel of diverse germplasm of alfalfa.
These are SNPs on two Illumina GoldenGate genotyping arrays and one 9k iSelect genotyping array.
Effort Versus Reward: Preparing samples for fungal community characterization in high-throughput sequencing surveys of soils
This data set consists of four data files. The FASTA file, Representative OTU sequences.fa, contains representative sequences from the operational taxonomic units (OTUs) shown in the OTU table. FASTA files can be opened in simple text editors, and sequences can be aligned using the BLAST tool (http://blast.ncbi.nlm.nih.gov/Blast.cgi) or open source software, like AliView (http://www.ormbunkar.se/aliview/). There are two Excel data files: OTU table and heatmaps.xlsx and Diversity Indexes.xlsx. The former contains the raw abundance data for the observed OTUs from the different experimental sites. The latter is a breakdown of various diversity indices that are grouped based on experimental characteristics, such as extraction volume, extraction method, etc. Excel_Archive.zip is a compressed version of the two Excel data files that have been converted to more archival-friendly formats using Excel Archival Tool.
The Air, Water, and Aquatic Environments (AWAE) research program is one of eight Science Program areas within the Rocky Mountain Research Station (RMRS). Our science develops core knowledge, methods, and technologies that enable effective watershed management in forests and grasslands, sustain biodiversity, and maintain healthy watershed conditions.
Microplitis demolitor (Hymenoptera: Braconidae) is a parasitoid used as a biological control agent to control larval-stage Lepidoptera and serves as a model for studying the function and evolution of symbiotic viruses in the genus Bracovirus. Using RNA-Seq data for this species and manual annotation of genes of viral origin, we annotated a high-quality gene set including 171 virus-derived protein-coding genes.
Data from: Clarireedia: A new fungal genus comprising four pathogenic species responsible for dollar spot disease of turfgrass
These datasets provide the phylogenetic evidence from three nucleotide sequence markers (CaM, ITS and Mcm7) that underlie the establishment of the new fungal genus Clarireedia, which includes four species that cause turfgrass dollar spot disease.