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Quantification of TAGs by LC-MS

    This Microsoft Excel workbook contains spreadsheets for calculating response-factor normalized triacylglycerol (TAG) compositions from response factors derived from GC-FID of fatty acid methyl esters (FAME). This workbook (1.160 Mb) reads the output from a ThermoScientific XCalibur Quan Browser •Excel Short Report• (In XCalibur: File • Export data to Excel • Export Short Excel report), and requires input of the FA composition determined by GC-FID (manually entered or read from Excel spreadsheet [provided upon request]).

    Data from: Agro-environmental consequences of shifting from nitrogen- to phosphorus-based manure management of corn.

      This experiment was designed to measure greenhouse gas (GHG) fluxes and related agronomic characteristics of a long-term corn-alfalfa rotational cropping system fertilized with manure (liquid versus semi-composted separated solids) from dairy animals. Different manure-application treatments were sized to fulfill two conditions: (1) an application rate to meet the agronomic soil nitrogen requirement of corn (“N-based” without manure incorporation, more manure), and (2) an application rate to match or to replace the phosphorus removal by silage corn from soils (“P-based” with incorporation, less manure). In addition, treatments tested the effects of liquid vs. composted-solid manure, and the effects of chemical nitrogen fertilizer. The controls consisted of non-manured inorganic N treatments (sidedress applications). These activities were performed during the 2014 and 2015 growing seasons as part of the Dairy Coordinated Agricultural Project, or Dairy CAP, as described below. The data from this experiment give insight into the factors controlling GHG emissions from similar cropping systems, and may be used for model calibration and validation after careful evaluation of the flagged data.