U.S. flag

An official website of the United States government

Annotations of Unigenes Assembled from Schizaphis graminum and Sipha flava

    Transcriptomes were assembled de novo from pools of adult aphids that were feeding on sorghum and switchgrass. Reads from all replicates were pooled, normalized in silico to 25X coverage, and assembled using Trinity. Only the most abundant isoform for each unigene was retained for annotation and unigenes with transcripts per million mapped reads (TPM) less than 0.5 were removed from the dataset. The remaining unigenes were annotated using Trinotate with BLASTP comparisons against the Swiss-Prot/UniProt database. In addition, Pfam-A assignments were computed using hmmer, signal peptide predictions were performed using SignalP, and transmembrane domain predictions were performed using tmHMM. Gene ontology (GO assignments) were retrieved from Trinotate using the highest scoring BLASTp matches as queries.

    Genomes To Fields 2016

      Phenotypic, genotypic, and environment data for the 2016 field season: The data is stored in [CyVerse](http://datacommons.cyverse.org/browse/iplant/home/shared/commons_repo/curated/GenomesToFields_G2F_2016_Data_Mar_2018).

      Genomes To Fields 2015

        Phenotypic, genotypic, and environment data for the 2015 field season: The data is stored in [CyVerse](http://datacommons.cyverse.org/browse/iplant/home/shared/commons_repo/curated/Carolyn_Lawrence_Dill_G2F_Mar_2017).

        Genomes To Fields 2014

          Phenotypic, genotypic, and environment data for the 2014 field season: The data is stored in [CyVerse](http://datacommons.cyverse.org/browse/iplant/home/shared/commons_repo/curated/Carolyn_Lawrence_Dill_G2F_Nov_2016_V.3).

          Genomes To Fields (G2F) Inbred Ear Imaging Data 2017

            A subset of ~30 inbreds were evaluated in 2014 and 2015 to develop an image based ear phenotyping tool. The data is stored in [CyVerse](http://datacommons.cyverse.org/browse/iplant/home/shared/commons_repo/curated/Edgar_Spalding_G2F_Inbred_Ear_Imaging_June_2017).

            Maize-GAMER: Maize B73 RefGen_v3 5b+

              This dataset from maize-GAMER is a new high-coverage and reproducible functional annotation of maize (*Zea mays*) protein coding genes based on Gene Ontology (GO) term assignments that covers all genes in the B73 RefGen_v3 5b+ set. Data are compressed gzip (.gz) files.

              Maize-GAMER: GO Annotations, Methods, Evaluation and Review

                maize-GAMER is a collaborative project to improve the status of gene functional annotation in maize (*Zea mays*). The project has three main areas of focus, namely * Design a pipeline for the functional annotation of maize genes. * Use manually curated test data to evaluate the annotations and generate a best subset of annotations for use * Design a user friendly review system for the community to provide feedback and endorsements of the annotations

                Data from: Shoot transcriptome of the giant reed, Arundo donax

                  The giant reed, *Arundo donax*, is a perennial grass species that has become an invasive plant in many countries. To establish a foundational molecular dataset, an llumina Hi-Seq protocol was used to sequence the transcriptome of actively growing shoots from an invasive genotype collected along the Rio Grande River, bordering Texas and Mexico. The assembly of 27,491 high confidence transcripts (≥200 bp) are reported with at least 70% coverage of known genes in other Poaceae species.