Data from: Cultivar resistance to common scab disease of potato is dependent on the pathogen species

All data from the paper "Cultivar resistance to common scab disease of potato is dependent on the pathogen species." Three separate datasets are included:
1.A csv file with the disease severity of three common scab pathogens across 55 different potato cultivars in a greenhouse pot assay (Figures 2-5 in the associated paper). The included R script was used with this data to perform the ANOVA for the data from the greenhouse pot assay (Table 2 in the associated paper). This script can be used in R for any similar dataset to calculate the significance and percent of total variation for any number of user-defined fixed effects.
2. A zipped file with all of the qPCR data for the expression of the txtAB genes (Figure 6 in the associated paper).
3. An Excel file with the HPLC data for making the thaxtomin detection standard curve and quantifying the amount of thaxtomin in the test sample.

Plants and Crops

Oncopeltus fasciatus hybrid genome assembly 1.0

The milkweed bug, Oncopeltus fasciatus, was sequenced as part of the i5k pilot project from Baylor College of Medicine (Illumina data). To augment those resources, we present here a hybrid genome assembly with low coverage PacBio data, assembled with PBJelly: the Oncopeltus fasciatus Hybrid Genome Assembly v1.0.

insects 5000 program

Data from: Genome of the small hive beetle (Aethina tumida, Coleoptera: Nitidulidae), a worldwide parasite of social bee colonies, provides insights into detoxification and herbivory

The small hive beetle (Aethina tumida, ATUMI) is an invasive parasite of bee colonies. ATUMI feeds on both fruits and bee nest products, facilitating its spread and increasing its impact on honey bees and other pollinators. The ATUMI genome has been sequenced and annotated, providing the first genomic resources for this species and for the Nitidulidae.

Genomics and Genetics

Data from: First Report of Squash vein yellowing virus in Watermelon in Guatemala

Watermelon (Citrullus lanatus) and other cucurbits are important crops grown in Guatemala for local consumption and export. Four samplings of crowns, peduncles, and/or leaves of symptomatic plants were made in March and April 2015. Total RNA was extracted from symptomatic plant tissue and tested by RT-PCR for SqVYV, CYSDV, PRSV, and/or begomoviruses. This is the first report of SqVYV infecting watermelon in Central America.

Plants and Crops

Data from: Genome-Wide Association Mapping of Loci Associated with Plant Growth and Forage Production under Salt Stress in Alfalfa (Medicago sativa L.)

Salinity tolerance is highly desirable to sustain alfalfa production in marginal lands that have been rendered saline. In this study, we used a diverse panel of 198 alfalfa accessions for mapping loci associated with plant growth and forage production under salt stress using genome-wide association studies (GWAS). The plants were genotyped using genotyping-by-sequencing (GBS). A greenhouse procedure was used for phenotyping four agronomic and physiological traits affected by salt stress, including dry weight (DW), plant height (PH), leaf chlorophyll content (LCC), and stomatal conductance (SC). For each trait, a stress susceptibility index (SSI) was used to evaluate plant performance under stressed and non-stressed conditions. Marker-trait association identified a total of 42 markers significantly associated with salt tolerance. They were located on all chromosomes except chromosome 2 based on the alignment of their flanking sequences to the reference genome (Medicago truncatula). Of those identified, 13 were associated with multiple traits. Several loci identified in the present study were also identified in previous reports. BLAST search revealed that 19 putative candidate genes linked to 24 significant markers. Among them, B3 DNA-binding protein, Thiaminepyrophosphokinase and IQ calmodulin-binding motif protein were identified among multiple traits in the present and previous studies. With further investigation, these markers and candidates would be useful for developing markers for marker-assisted selection in breeding programs to improve alfalfa cultivars with enhanced tolerance to salt stress.

Genomics and Genetics