Honey bee foraging activity in four soybean fields during the blooming period (R2-R3), as well as before or after bloom, was recorded continuously using Sony ICD-PX370 audio recorders affixed to a wooden stake and placed below the soybean canopy, at the height of the highest flowers, approximately 35 m from the field edge.

We present the annotation results of 15 arthropod proteomes using an open source, open access and containerized pipeline for genome-scale functional annotation of insect proteomes and apply it to a diverse range of arthropod species.

[Note: This dataset is superseded by Version 2, https://doi.org/10.15482/USDA.ADC/1521354 ] Pollinator refuges such as wildflower strips are planted on farms with the goals of mitigating wild pollinator declines and promoting crop pollination services. It is unclear, however, whether or how these goals are impacted by managed honey bee (Apis mellifera L.) hives on farms. We examined how wildflower strips and honey bee hives and/or their interaction influence wild bee communities and the fruit count of two pollinator-dependent crops across 21 farms in the Mid-Atlantic U.S.

The small hive beetle (SHB) *Aethina tumida* is an opportunistic parasite that feeds on bee larvae, honey, and pollen. To identify sets of beetle microbes and the transmission of microbes from bees to beetles, a metagenomic analysis was performed. We identified sets of herbivore‐associated bacteria, as well as typical bee symbiotic bacteria for pollen digestion, in SHB larvae and adults. Microbes found from Illumina DNA reads using Kraken include DNA reads aligned to HoloBee Bar database report, adult beetle microbe qPCR results from DNA extraction, adult beetle de novo assembled contig_names, larvae beetle Pacbio_reads.