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Germplasm Resources Information Network (GRIN)

    The Germplasm Resources Information Network (GRIN) is an online portal for information about agricultural genetic resources that are managed by the Agricultural Research Service of USDA, along with U.S. partnering organizations.

    2014 Swine CAFO Study SE for Agricultural Antibiotic Resistance in Mississippi State, Mississippi

      The purpose of this study was to measure the microbial constituents of swine-sow, -nursery, and -finisher farm manure lagoon wastewater and determine the changes induced by farm management. A total of 37 farms were visited in the Mid-South USA and analyzed for the genes 16S rRNA, spaQ (Salmonella spp.), Camp-16S (Campylobacter spp.), tetA, tetB, ermF, ermA, mecA, and intI using quantitative PCR. Additionally, 16S rRNA sequence libraries were created.

      Data from: The assembled transcriptome of the adult horn fly, Haematobia irritans

        To better understand the adult horn fly, *Haematobia irritans irritans*, and the development of resistance in natural populations, an Illumina paired-end read HiSeq and GAII approach was used to determine the transcriptomes of untreated control adult females, untreated control adult males, permethrin-treated surviving adult males and permethrin + piperonyl butoxide-treated killed adult males from a Louisiana population of horn flies with a moderate level of pyrethroid resistance.

        The GRIN-Global Project

          GRIN-Global is an ongoing international collaborative project to develop shared and open-source applications that help manage plant germplasm collections.

          Data from: Pyrosequencing-Based Analysis of the Microbiome Associated with the Horn Fly, Haematobia irritans

            The bacterial 16S tag-encoded FLX-titanium amplicon pyrosequencing (bTEFAP) method was used to carry out the classification analysis of bacterial flora in adult female and male horn flies and horn fly eggs. The bTEFAP method identified 16S rDNA sequences in our samples which allowed the identification of various prokaryotic taxa associated with the life stage examined. This is the first comprehensive report of bacterial flora associated with the horn fly using a culture-independent method. Several rumen, environmental, symbiotic and pathogenic bacteria associated with the horn fly were identified and quantified. This is the first report of the presence of Wolbachia in horn flies of USA origin and is the first report of the presence of Rikenella in an obligatory blood feeding insect.

            Germplasm Resources Information Network (GRIN)

              The Germplasm Resources Information Network (GRIN) is an online portal for information about agricultural genetic resources that are managed by the Agricultural Research Service of USDA, along with U.S. partnering organizations.