Culicoides biting midges are important vectors of diverse microbes such as viruses, protozoa, and nematodes that cause diseases in wild and domestic animals. To investigate the role of microbial communities in midge larval habitat utilization in the wild, we characterized microbial communities (bacterial, protistan, fungal and metazoan) in soils from disturbed (bison and cattle grazed) and undisturbed (non-grazed) pond and spring potential midge larval habitats. We evaluated the influence of habitat and grazing disturbance and their interaction on microbial communities, diversity, presence of midges, and soil properties.

USDA-ARS REAP Study (Ithaca, NE) - NEMEIRR Sustainable intensification of high-yielding production systems may help meet increasing demands for food, fuel, and fiber worldwide. Specifically, corn stover is being removed by producers for livestock purposes, and stover is also targeted as a primary 2nd generation biofuel feedstock. The NEMEIRR experimental objectives are to quantify how stover removal (no removal, moderate removal, high removal) and tillage management (no-till, disk) affect crop yields, soil organic carbon, soil greenhouse gas emissions, and other soil responses (microbial community structure, function; soil health). This experiment is conducted in a fully irrigated continuous corn system in the western Corn Belt, and soil and plant measurements have been taken since study establishment in 2001.

Chondroitin sulfate (CS) has been widely used for medical and nutraceutical purposes due to its roles in maintaining tissue structural integrity. We investigated if CS disaccharides may act as a bioactive compound and modulate gut microbial composition in mice. Our data show that CS disaccharides supplementation for 16 days significantly reduced blood LPS in the mice experiencing exhaustive exercise stress. CS disaccharides partially restored total fecal short-chain fatty acids from the level significantly repressed in mice under the stress. Our findings demonstrated that CS was likely butyrogenic and resulted in a significant increase in fecal butyrate concentration. CS disaccharides had a profound impact on gut microbial composition, affecting the abundance of 13.6% and 7.3% Operational Taxonomic Units in fecal microbial communities in healthy and stressed mice, respectively. CS disaccharides reduced the prevalence of inflammatory Proteobacteria. Together, our findings demonstrated that CS may ameliorate stress-induced intestinal inflammation. Furthermore, CS significantly increased intestinal *Bacteroides acidifaciens* population, indirectly exerting its immunomodulatory effect on the intestine. CS disaccharides had a significant impact on a broad range of biological pathways under stressed condition, such as ABC transporters, two-component systems, and carbohydrate metabolism. Our results will facilitate the development of CS as a bioactive nutraceutical.