The overall goal of the Carbon Crop study, established in 2000, was to assess strategies for increasing soil C sequestration including converting to no till systems and including perennial grasses (e.g., switchgrass and big bluestem) Overall, the goal of the study has remained constant, although individual treatments were changed after an incremental soil sampling, in response to new hypotheses and questions.

Transcriptomes were assembled de novo from pools of adult aphids that were feeding on sorghum and switchgrass. Reads from all replicates were pooled, normalized in silico to 25X coverage, and assembled using Trinity. Only the most abundant isoform for each unigene was retained for annotation and unigenes with transcripts per million mapped reads (TPM) less than 0.5 were removed from the dataset. The remaining unigenes were annotated using Trinotate with BLASTP comparisons against the Swiss-Prot/UniProt database. In addition, Pfam-A assignments were computed using hmmer, signal peptide predictions were performed using SignalP, and transmembrane domain predictions were performed using tmHMM. Gene ontology (GO assignments) were retrieved from Trinotate using the highest scoring BLASTp matches as queries.

Feedstock readiness level evaluations are performed for a specific feedstock-conversion process combination and for a particular region. FSRL evaluations complement evaluations of Fuel Readiness Level (FRL) and environmental progress. The data from this evaluation, compiled in June 2018, assesses the maturity of *Panicum virgatum* (switchgrass) as a feedstock for the Alcohol-to-Jet conversion process in the United States Southeast region.

The Agricultural Land Management Alternative with Numerical Assessment Criteria (ALMANAC) model simulates crop growth, competition, light interception by leaves, biomass accumulation, partitioning of biomass into grain, water use, nutrient uptake, and growth constraints such as water, temperature, and nutrient stress. Plant development is temperature driven, with duration of growth stages dependent on degree days. Each plant species has a defined base temperature and optimum temperature.