The Aquatic eDNAtlas Project: Lab Results Map - USFS RMRS

The eDNA samples in the eDNAtlas database describe species occurrence locations and were collected by the U.S. Forest Service and numerous agencies that have partnered with the National Genomics Center for Wildlife and Fish Conservation (NGC) throughout the United States. The eDNAtlas is accessed via an interactive ArcGIS Online (AGOL) map that allows users to view and download sample site information and lab results of species occurrence for the U.S. The results are primarily based on samples analyzed at the National Genomics Center for Wildlife and Fish Conservation (NGC) and associated with geospatial attributes created by the Boise Spatial Streams Group (BSSG).

Genomics and Genetics

The Range-Wide Bull Trout eDNA Project - USFS RMRS

The bull trout (Salvelinus confluentus) eDNA survey results Online Map allows users to view the survey results in an interactive map by coupling 1) predictions from the range-wide, spatially precise Climate Shield model on the location of natal habitats of bull trout with 2) a sampling template for every 8-digit hydrologic unit in the historical range of bull trout, based on the probability of detecting bull trout presence using environmental DNA (eDNA) sampling. The map provides the ability to zoom in and look at an area of interest, as well as to create queries or select an area to download points as a shapefile.

Genomics and Genetics

De novo transcriptome assembly and annotations for wheat curl mite (Aceria tosichella)

To study the impact of wheat streak mosaic virus on global gene expression in wheat curl mite, we generated a de novo transcriptome assembly using 50 x 50 paired end reads from the Illumina HiSeq 2500. Reads were assembled using Trinity (version 2.0.6) and contigs greater than 200 nt were retained. All assembled transcripts were annotated using the Trinotate pipeline using blastp searches against the Swiss-prot/Uni-Prot database, blastx searches against the Swiss-prot/Uni-Prot databases, HMM searches against the Pfam-A database, blastp searches against the non-redundant protein database, and signalP and tmHMM predictions. To reduce noise from low abundance transcripts not well supported by the data, we filtered the assembly to retain only those transcripts with TPM values >=0.5.

Genomics and Genetics

Annotations of Unigenes Assembled from Schizaphis graminum and Sipha flava

Transcriptomes were assembled de novo from pools of adult aphids that were feeding on sorghum and switchgrass. Reads from all replicates were pooled, normalized in silico to 25X coverage, and assembled using Trinity. Only the most abundant isoform for each unigene was retained for annotation and unigenes with transcripts per million mapped reads (TPM) less than 0.5 were removed from the dataset. The remaining unigenes were annotated using Trinotate with BLASTP comparisons against the Swiss-Prot/UniProt database. In addition, Pfam-A assignments were computed using hmmer, signal peptide predictions were performed using SignalP, and transmembrane domain predictions were performed using tmHMM. Gene ontology (GO assignments) were retrieved from Trinotate using the highest scoring BLASTp matches as queries.

Genomics and Genetics

Orussus abietinus Official Gene Set OGSv1.0

The Orussus abietinus genome was recently sequenced and annotated as part of the i5k pilot project by the Baylor College of Medicine. The Orussus abietinus research community has manually reviewed and curated the computational gene predictions and generated an official gene set, OGSv1.0. The general procedure for generating this OGS is outlined here: https://github.com/NAL-i5K/I5KNAL_OGS/wiki. OGSv1.0 was generated by merging gene set OABI-V0.5.3-Models generated by the Baylor College of Medicine, and community-curated models in the Apollo software, after QC of the Apollo output.

insects 5000 program

Athalia rosae Official Gene Set OGSv1.0

The Athalia rosae genome was recently sequenced and annotated as part of the i5k pilot project by the Baylor College of Medicine. The Athalia rosae research community has manually reviewed and curated the computational gene predictions and generated an official gene set, OGSv1.0. The general procedure for generating this OGS is outlined here: https://github.com/NAL-i5K/I5KNAL_OGS/wiki. OGSv1.0 was generated by merging gene set AROS-V0.5.3-Models generated by the Baylor College of Medicine, and community-curated models in the Apollo software, after QC of the Apollo output.

insects 5000 program

Leptinotarsa decemlineata Official Gene set v1.2

The Leptinotarsa decemlineata genome was recently sequenced and annotated as part of the i5k pilot project by the Baylor College of Medicine. The L. decemlineata research community has manually reviewed and curated the computational gene predictions and generated an official gene set, OGSv1.2. OGSv1.1 is an integration of automatic gene predictions from Maker (performed by Dan Hughes at Baylor College of Medicine) with manual annotations by the research community (done via the Apollo manual annotation software). The coordinates of OGSv1.1 were converted to the latest genome assembly, GCF_000500325.1, using coordinates_conversion and remap-gff3, to generate OGSv1.2.

insects 5000 program