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Comparison of methods to detect low levels of Salmonella enterica in surface waters to support antimicrobial resistance surveillance efforts performed in multiple laboratories

    Identifying and developing effective and sensitive detection methods for antimicrobial resistant Salmonella enterica from surface water is a goal of the U.S. National Antimicrobial Resistance Monitoring System (NARMS). No specific microbiological methods used in surveillance efforts for Salmonella enterica or antimicrobial resistant S. enterica in water have been standardized or reported in the U.S. Here we describe a multi-laboratory evaluation of four methods, bulk water enrichment (BW), vertical Modified Moore Swab (VMMS), modified Standard Method 9260.B3 (SM), and dead-end ultrafiltration (DEUF), to recover S. enterica from surface water.

    Evapotranspiration, Irrigation, Dew/frost - Water Balance Data for The Bushland, Texas Soybean Datasets

      This dataset consist of 15-minute and daily amounts of evapotranspiration (ET), dew/frost fall, precipitation (rain/snow), irrigation, scale counterweight adjustment, and emptying of drainage tanks, all in mm. The values are the result of a rigorous quality control process involving algorithms for detecting dew/frost accumulations, and precipitation (rain and snow). Changes in lysimeter mass due to emptying of drainage tanks, counterweight adjustment, maintenance activity, and harvest are accounted for such that ET values are minimally affected. Data are for soybean [*Glycine max* (L.) Merr.] grown at the USDA-ARS Conservation and Production Laboratory (CPRL), Soil and Water Management Research Unit (SWMRU), Bushland, Texas (Lat. 35.186714°, Long. -102.094189°, elevation 1170 m above MSL) in 1995, 2003, 2004, 2010 and 2019 on large, precision weighing lysimeters, each in the center of a 4.44 ha square field.

      The Bushland, Texas Soybean Datasets

        This parent dataset links to five seasons of datasets on soybean [Glycine max (L.) Merr.] grown for seed at the USDA-ARS Conservation and Production Laboratory (CPRL), Soil and Water Management Research Unit (SWMRU), Bushland, Texas (Lat. 35.186714°, Long. -102.094189°, elevation 1170 m above MSL) in 1995, 2003, 2004, 2010, and 2019. In 1995, 2003, 2004, and 2010, soybean was grown on two large, precision weighing lysimeters, each in the center of a 4.44 ha square field. In 2019, soybean was grown on four large, precision weighing lysimeters and on the 4.44 ha square fields surrounding each lysimeter. The datasets for individual season years consist of soil water content, weather, crop growth and yield, agronomic calendar, water balance (evapotranspiration, precipitation, dew/frost, irrigation), and lysimeter energy and water balance data.

        The Bushland, Texas Sunflower Datasets

          This parent dataset links to two seasons of datasets on sunflower grown for seed at the USDA-ARS Conservation and Production Laboratory (CPRL), Soil and Water Management Research Unit (SWMRU), Bushland, Texas (Lat. 35.186714°, Long. -102.094189°, elevation 1170 m above MSL) in 2009, and 2011. Sunflower was grown on two large, precision weighing lysimeters, each in the center of a 4.44 ha square field. The datasets for individual season years consist of soil water content, weather, crop growth and yield, agronomic calendar, water balance (evapotranspiration, precipitation, dew/frost, irrigation), and lysimeter energy and water balance data.

          Evapotranspiration, Irrigation, Dew/frost - Water Balance Data for The Bushland, Texas Sunflower Datasets

            This dataset consist of 15-minute and daily amounts of evapotranspiration (ET), dew/frost fall, precipitation (rain/snow), irrigation, scale counterweight adjustment, and emptying of drainage tanks, all in mm. The values are the result of a rigorous quality control process involving algorithms for detecting dew/frost accumulations, and precipitation (rain and snow). Changes in lysimeter mass due to emptying of drainage tanks, counterweight adjustment, maintenance activity, and harvest are accounted for such that ET values are minimally affected. Data are for sunflower grown at the USDA-ARS Conservation and Production Laboratory (CPRL), Soil and Water Management Research Unit (SWMRU), Bushland, Texas (Lat. 35.186714°, Long. -102.094189°, elevation 1170 m above MSL) in 2009 and 2022. Sunflower was grown for seed on two large, precision weighing lysimeters, each in the center of a 4.44 ha square field.

            Evapotranspiration, Irrigation, Dew/frost - Water Balance Data for The Bushland, Texas Winter Wheat Datasets

              This dataset consist of 15-minute and daily amounts of evapotranspiration (ET), dew/frost fall, precipitation (rain/snow), irrigation, scale counterweight adjustment, and emptying of drainage tanks, all in mm. The values are the result of a rigorous quality control process involving algorithms for detecting dew/frost accumulations, and precipitation (rain and snow). Changes in lysimeter mass due to emptying of drainage tanks, counterweight adjustment, maintenance activity, and harvest are accounted for such that ET values are minimally affected. Data are for winter wheat grown at the USDA-ARS Conservation and Production Laboratory (CPRL), Soil and Water Management Research Unit (SWMRU), Bushland, Texas (Lat. 35.186714°, Long. -102.094189°, elevation 1170 m above MSL) in the 1989-1990, 1991-1992, and 1992-1993 seasons. Winter wheat was grown on two large, precision weighing lysimeters, each in the center of a 4.44 ha square field.

              Standard Quality Controlled Research Weather Data – USDA-ARS, Bushland, Texas

                The dataset contains 15-minute weather data from the USDA-ARS Conservation and Production Laboratory (CPRL), Soil and Water Management Research Unit (SWMRU) research weather station, Bushland, Texas (Lat. 35.186714°, Long. -102.094189°, elevation 1170 m above MSL). The data are from sensors deployed at standard heights over grass that is irrigated and mowed during the growing season to reference evapotranspiration standards.

                The Bushland, Texas, Winter Wheat Datasets

                  This parent dataset links to three seasons of datasets on winter wheat grown for grain at the USDA-ARS Conservation and Production Laboratory (CPRL), Soil and Water Management Research Unit (SWMRU), Bushland, Texas (Lat. 35.186714°, Long. -102.094189°, elevation 1170 m above MSL) in 1989-90, 1991-92, and 1992-93. Winter wheat was grown on two large, precision weighing lysimeters, each in the center of a 4.44 ha square field. The datasets for individual years consist of soil water content, weather, crop growth and yield, agronomic calendar, water balance (evapotranspiration, precipitation, dew/frost, irrigation), and lysimeter energy and water balance data. Additional resources provide metadata on conventions, symbols used, geographic coordinates of fields, lysimeters and weather stations, and soil properties.

                  Evapotranspiration, Irrigation, Dew/frost - Water Balance Data for The Bushland, Texas Alfalfa Datasets

                    This dataset consist of 15-minute and daily amounts of evapotranspiration (ET), dew/frost fall, precipitation (rain/snow), irrigation, scale counterweight adjustment, and emptying of drainage tanks, all in mm. The values are the result of a rigorous quality control process involving algorithms for detecting dew/frost accumulations, and precipitation (rain and snow). Changes in lysimeter mass due to emptying of drainage tanks, counterweight adjustment, maintenance activity, and harvest are accounted for such that ET values are minimally affected. Data are for alfalfa grown at the USDA-ARS Conservation and Production Laboratory (CPRL), Soil and Water Management Research Unit (SWMRU), Bushland, Texas (Lat. 35.186714°, Long. -102.094189°, elevation 1170 m above MSL) in 1996 through 1999. Alfalfa was grown on two large, precision weighing lysimeters, each in the center of a 4.44 ha square field.