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Comparison of methods to detect low levels of Salmonella enterica in surface waters to support antimicrobial resistance surveillance efforts performed in multiple laboratories

    Identifying and developing effective and sensitive detection methods for antimicrobial resistant Salmonella enterica from surface water is a goal of the U.S. National Antimicrobial Resistance Monitoring System (NARMS). No specific microbiological methods used in surveillance efforts for Salmonella enterica or antimicrobial resistant S. enterica in water have been standardized or reported in the U.S. Here we describe a multi-laboratory evaluation of four methods, bulk water enrichment (BW), vertical Modified Moore Swab (VMMS), modified Standard Method 9260.B3 (SM), and dead-end ultrafiltration (DEUF), to recover S. enterica from surface water.

    The Bushland, Texas Sunflower Datasets

      This parent dataset links to two seasons of datasets on sunflower grown for seed at the USDA-ARS Conservation and Production Laboratory (CPRL), Soil and Water Management Research Unit (SWMRU), Bushland, Texas (Lat. 35.186714°, Long. -102.094189°, elevation 1170 m above MSL) in 2009, and 2011. Sunflower was grown on two large, precision weighing lysimeters, each in the center of a 4.44 ha square field. The datasets for individual season years consist of soil water content, weather, crop growth and yield, agronomic calendar, water balance (evapotranspiration, precipitation, dew/frost, irrigation), and lysimeter energy and water balance data.

      Evapotranspiration, Irrigation, Dew/frost - Water Balance Data for The Bushland, Texas Sunflower Datasets

        This dataset consist of 15-minute and daily amounts of evapotranspiration (ET), dew/frost fall, precipitation (rain/snow), irrigation, scale counterweight adjustment, and emptying of drainage tanks, all in mm. The values are the result of a rigorous quality control process involving algorithms for detecting dew/frost accumulations, and precipitation (rain and snow). Changes in lysimeter mass due to emptying of drainage tanks, counterweight adjustment, maintenance activity, and harvest are accounted for such that ET values are minimally affected. Data are for sunflower grown at the USDA-ARS Conservation and Production Laboratory (CPRL), Soil and Water Management Research Unit (SWMRU), Bushland, Texas (Lat. 35.186714°, Long. -102.094189°, elevation 1170 m above MSL) in 2009 and 2022. Sunflower was grown for seed on two large, precision weighing lysimeters, each in the center of a 4.44 ha square field.

        Evapotranspiration, Irrigation, Dew/frost - Water Balance Data for The Bushland, Texas Winter Wheat Datasets

          This dataset consist of 15-minute and daily amounts of evapotranspiration (ET), dew/frost fall, precipitation (rain/snow), irrigation, scale counterweight adjustment, and emptying of drainage tanks, all in mm. The values are the result of a rigorous quality control process involving algorithms for detecting dew/frost accumulations, and precipitation (rain and snow). Changes in lysimeter mass due to emptying of drainage tanks, counterweight adjustment, maintenance activity, and harvest are accounted for such that ET values are minimally affected. Data are for winter wheat grown at the USDA-ARS Conservation and Production Laboratory (CPRL), Soil and Water Management Research Unit (SWMRU), Bushland, Texas (Lat. 35.186714°, Long. -102.094189°, elevation 1170 m above MSL) in the 1989-1990, 1991-1992, and 1992-1993 seasons. Winter wheat was grown on two large, precision weighing lysimeters, each in the center of a 4.44 ha square field.

          Standard Quality Controlled Research Weather Data – USDA-ARS, Bushland, Texas

            The dataset contains 15-minute weather data from the USDA-ARS Conservation and Production Laboratory (CPRL), Soil and Water Management Research Unit (SWMRU) research weather station, Bushland, Texas (Lat. 35.186714°, Long. -102.094189°, elevation 1170 m above MSL). The data are from sensors deployed at standard heights over grass that is irrigated and mowed during the growing season to reference evapotranspiration standards.

            The Bushland, Texas, Winter Wheat Datasets

              This parent dataset links to three seasons of datasets on winter wheat grown for grain at the USDA-ARS Conservation and Production Laboratory (CPRL), Soil and Water Management Research Unit (SWMRU), Bushland, Texas (Lat. 35.186714°, Long. -102.094189°, elevation 1170 m above MSL) in 1989-90, 1991-92, and 1992-93. Winter wheat was grown on two large, precision weighing lysimeters, each in the center of a 4.44 ha square field. The datasets for individual years consist of soil water content, weather, crop growth and yield, agronomic calendar, water balance (evapotranspiration, precipitation, dew/frost, irrigation), and lysimeter energy and water balance data. Additional resources provide metadata on conventions, symbols used, geographic coordinates of fields, lysimeters and weather stations, and soil properties.

              Evapotranspiration, Irrigation, Dew/frost - Water Balance Data for The Bushland, Texas Alfalfa Datasets

                This dataset consist of 15-minute and daily amounts of evapotranspiration (ET), dew/frost fall, precipitation (rain/snow), irrigation, scale counterweight adjustment, and emptying of drainage tanks, all in mm. The values are the result of a rigorous quality control process involving algorithms for detecting dew/frost accumulations, and precipitation (rain and snow). Changes in lysimeter mass due to emptying of drainage tanks, counterweight adjustment, maintenance activity, and harvest are accounted for such that ET values are minimally affected. Data are for alfalfa grown at the USDA-ARS Conservation and Production Laboratory (CPRL), Soil and Water Management Research Unit (SWMRU), Bushland, Texas (Lat. 35.186714°, Long. -102.094189°, elevation 1170 m above MSL) in 1996 through 1999. Alfalfa was grown on two large, precision weighing lysimeters, each in the center of a 4.44 ha square field.

                The Bushland, Texas, Alfalfa Datasets

                  This parent dataset links to four seasons of datasets on alfalfa grown for grain at the USDA-ARS Conservation and Production Laboratory (CPRL), Soil and Water Management Research Unit (SWMRU), Bushland, Texas (Lat. 35.186714°, Long. -102.094189°, elevation 1170 m above MSL) in 1996 through 1999. Alfalfa was grown on two large, precision weighing lysimeters, each in the center of a 4.44 ha square field. The datasets for individual years consist of soil water content, weather, crop growth and yield, agronomic calendar, water balance (evapotranspiration, precipitation, dew/frost, irrigation), and lysimeter energy and water balance data. Additional resources provide metadata on conventions, symbols used, geographic coordinates of fields, lysimeters and weather stations, and soil properties.

                  Data from: Pasture dragging fails to reliably suppress the emergence of horn flies (Haematobia irritans) and face flies (Musca autumnalis) from dung pats in a Mid-Atlantic North American climate

                    In order to determine if pasture dragging suppresses the emergence of horn flies (Haematobia irritans) and face flies (Musca autumnalis) from dung pats in a Mid-Atlantic North American climate, bovine dung pats were spread with pasture drag or plaster knife and covered with emergence traps, and emerging flies quantified.

                    Evapotranspiration, Irrigation, Dew/frost - Water Balance Data for The Bushland, Texas Maize for Grain Datasets

                      This dataset consist of 15-minute and daily amounts of evapotranspiration (ET), dew/frost fall, precipitation (rain/snow), irrigation, scale counterweight adjustment, and emptying of drainage tanks, all in mm. The values are the result of a rigorous quality control process involving algorithms for detecting dew/frost accumulations, and precipitation (rain and snow). Changes in lysimeter mass due to emptying of drainage tanks, counterweight adjustment, maintenance activity, and harvest are accounted for such that ET values are minimally affected. Data are for maize grown for grain at the USDA-ARS Conservation and Production Laboratory (CPRL), Soil and Water Management Research Unit (SWMRU), Bushland, Texas (Lat. 35.186714°, Long. -102.094189°, elevation 1170 m above MSL) for 1989, 1990, 1994, 2013, 2016, and 2018. Maize was grown on four large, precision weighing lysimeters, each in the center of a 4.44 ha square field.