U.S. flag

An official website of the United States government

The Range-Wide Bull Trout eDNA Project - USFS RMRS

    The bull trout (*Salvelinus confluentus*) eDNA survey results Online Map allows users to view the survey results in an interactive map by coupling 1) predictions from the range-wide, spatially precise Climate Shield model on the location of natal habitats of bull trout with 2) a sampling template for every 8-digit hydrologic unit in the historical range of bull trout, based on the probability of detecting bull trout presence using environmental DNA (eDNA) sampling. The map provides the ability to zoom in and look at an area of interest, as well as to create queries or select an area to download points as a shapefile.

    Annotations of Unigenes Assembled from Schizaphis graminum and Sipha flava

      Transcriptomes were assembled de novo from pools of adult aphids that were feeding on sorghum and switchgrass. Reads from all replicates were pooled, normalized in silico to 25X coverage, and assembled using Trinity. Only the most abundant isoform for each unigene was retained for annotation and unigenes with transcripts per million mapped reads (TPM) less than 0.5 were removed from the dataset. The remaining unigenes were annotated using Trinotate with BLASTP comparisons against the Swiss-Prot/UniProt database. In addition, Pfam-A assignments were computed using hmmer, signal peptide predictions were performed using SignalP, and transmembrane domain predictions were performed using tmHMM. Gene ontology (GO assignments) were retrieved from Trinotate using the highest scoring BLASTp matches as queries.

      Data from: Characterization of Adult Transcriptomes from the Omnivorous Lady Beetle Coleomegilla maculata Fed Pollen or Insect Egg Diet

        Expressed genes from two individual sibling specimens of *Coleomegilla maculata* (Coleoptera: Coccinellidae). One individual was fed only insect eggs as an adult, and one was fed only pollen as an adult. Two sequenced samples, total RNA from a single individual adult specimen of *Coleomegilla maculata*, a beneficial lady beetle common in agroecosystems and native to North America. One sample was an adult fed only insect eggs (carnivore diet) and one sample was an adult fed only pollen (plant-based diet); insects were reared from the same egg mass (siblings), fed identical diet while in larval stage.