The Range-Wide Bull Trout eDNA Project - USFS RMRS

The bull trout (Salvelinus confluentus) eDNA survey results Online Map allows users to view the survey results in an interactive map by coupling 1) predictions from the range-wide, spatially precise Climate Shield model on the location of natal habitats of bull trout with 2) a sampling template for every 8-digit hydrologic unit in the historical range of bull trout, based on the probability of detecting bull trout presence using environmental DNA (eDNA) sampling. The map provides the ability to zoom in and look at an area of interest, as well as to create queries or select an area to download points as a shapefile.

Genomics and Genetics

Annotations of Unigenes Assembled from Schizaphis graminum and Sipha flava

Transcriptomes were assembled de novo from pools of adult aphids that were feeding on sorghum and switchgrass. Reads from all replicates were pooled, normalized in silico to 25X coverage, and assembled using Trinity. Only the most abundant isoform for each unigene was retained for annotation and unigenes with transcripts per million mapped reads (TPM) less than 0.5 were removed from the dataset. The remaining unigenes were annotated using Trinotate with BLASTP comparisons against the Swiss-Prot/UniProt database. In addition, Pfam-A assignments were computed using hmmer, signal peptide predictions were performed using SignalP, and transmembrane domain predictions were performed using tmHMM. Gene ontology (GO assignments) were retrieved from Trinotate using the highest scoring BLASTp matches as queries.

Genomics and Genetics

Data from: Characterization of Adult Transcriptomes from the Omnivorous Lady Beetle Coleomegilla maculata Fed Pollen or Insect Egg Diet

Expressed genes from two individual sibling specimens of Coleomegilla maculata (Coleoptera: Coccinellidae). One individual was fed only insect eggs as an adult, and one was fed only pollen as an adult. Two sequenced samples, total RNA from a single individual adult specimen of Coleomegilla maculata, a beneficial lady beetle common in agroecosystems and native to North America. One sample was an adult fed only insect eggs (carnivore diet) and one sample was an adult fed only pollen (plant-based diet); insects were reared from the same egg mass (siblings), fed identical diet while in larval stage.

Data from: Defensive aphid symbiont Hamiltonella defensa effects on Aphelinus glycinis and Aphelinus atriplicis

Aphelinus glycinis was collected in the Peoples Republic of China under a Memorandum of Understanding between their Ministry of Agriculture and the United States Department of Agriculture (USDA). Aphelinus atriplicis was collected by employees of the USDA, Agricultural Research Service (ARS), in the Republic of Georgia with the permission of that government. The parasitoids were imported into the USDA, ARS, Beneficial Insect Introductions Research Unit containment facility in Newark, Delaware, under permits from the USDA, Animal and Plant Health Inspection Service (Permit Numbers P526P-08-02142 and P526P-09-01929). No specific permissions were required to collect Aphis craccivora or Acyrthosiphon pisum because these are cosmopolitan aphids that occur in the field throughout North America. None of the species collected or studied are endangered or protected.

Agroecosystems & Environment