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Data from: Sporobolus stapfianus: Insights into desiccation tolerance in the resurrection grasses from linking transcriptomics to metabolomics

    Non-targeted metabolomics, combined with transcriptomics via a NimbleGen array platform, are used to study how gene expression and metabolite profiles can be linked to generate a more detailed mechanistic appreciation of the cellular response to both desiccation and rehydration in the C4 resurrection grass, *Sporobolus stapfianus* Gandoger, as a member of a group of important forage grasses.

    De novo transcriptome assembly and annotations for wheat curl mite (Aceria tosichella)

      To study the impact of wheat streak mosaic virus on global gene expression in wheat curl mite, we generated a de novo transcriptome assembly using 50 x 50 paired end reads from the Illumina HiSeq 2500. Reads were assembled using Trinity (version 2.0.6) and contigs greater than 200 nt were retained. All assembled transcripts were annotated using the Trinotate pipeline using blastp searches against the Swiss-prot/Uni-Prot database, blastx searches against the Swiss-prot/Uni-Prot databases, HMM searches against the Pfam-A database, blastp searches against the non-redundant protein database, and signalP and tmHMM predictions. To reduce noise from low abundance transcripts not well supported by the data, we filtered the assembly to retain only those transcripts with TPM values >=0.5.

      Annotations of Unigenes Assembled from Schizaphis graminum and Sipha flava

        Transcriptomes were assembled de novo from pools of adult aphids that were feeding on sorghum and switchgrass. Reads from all replicates were pooled, normalized in silico to 25X coverage, and assembled using Trinity. Only the most abundant isoform for each unigene was retained for annotation and unigenes with transcripts per million mapped reads (TPM) less than 0.5 were removed from the dataset. The remaining unigenes were annotated using Trinotate with BLASTP comparisons against the Swiss-Prot/UniProt database. In addition, Pfam-A assignments were computed using hmmer, signal peptide predictions were performed using SignalP, and transmembrane domain predictions were performed using tmHMM. Gene ontology (GO assignments) were retrieved from Trinotate using the highest scoring BLASTp matches as queries.

        Data from: Generation and analysis of blueberry transcriptome sequences from leaves, developing fruit, and flower buds from cold acclimation through deacclimation

          There has been increased consumption of blueberries in recent years fueled in part because of their many recognized health benefits. Blueberry fruit is very high in anthocyanins, which have been linked to improved night vision, prevention of macular degeneration, anti-cancer activity, and reduced risk of heart disease. Very few genomic resources have been available for blueberry, however. Further development of genomic resources like expressed sequence tags (ESTs), molecular markers, and genetic linkage maps could lead to more rapid genetic improvement. Marker-assisted selection could be used to combine traits for climatic adaptation with fruit and nutritional quality traits.

          Data from: Characterization of Adult Transcriptomes from the Omnivorous Lady Beetle Coleomegilla maculata Fed Pollen or Insect Egg Diet

            Expressed genes from two individual sibling specimens of *Coleomegilla maculata* (Coleoptera: Coccinellidae). One individual was fed only insect eggs as an adult, and one was fed only pollen as an adult. Two sequenced samples, total RNA from a single individual adult specimen of *Coleomegilla maculata*, a beneficial lady beetle common in agroecosystems and native to North America. One sample was an adult fed only insect eggs (carnivore diet) and one sample was an adult fed only pollen (plant-based diet); insects were reared from the same egg mass (siblings), fed identical diet while in larval stage.

            Data from: The assembled transcriptome of the adult horn fly, Haematobia irritans

              To better understand the adult horn fly, *Haematobia irritans irritans*, and the development of resistance in natural populations, an Illumina paired-end read HiSeq and GAII approach was used to determine the transcriptomes of untreated control adult females, untreated control adult males, permethrin-treated surviving adult males and permethrin + piperonyl butoxide-treated killed adult males from a Louisiana population of horn flies with a moderate level of pyrethroid resistance.

              Data from: Chromosome-level genome assembly and transcriptome of the green alga Chromochloris zofingiensis illuminates astaxanthin production

                For genome assembly of *C. zofingiensis* strain SAG 211–14, we used a hybrid approach blending short reads (Illumina), long reads (Pacific Biosciences of California), and whole-genome optical mapping (OpGen) (SI Appendix, SI Text and Datasets S1–S19, and refer to SI Appendix, Datasets Key). The combined power of these approaches yielded a high-quality haploid nuclear genome of *C. zofingiensis* of ∼58 Mbp distributed over 19 chromosomes (Fig. 2) in the tradition of model organism projects, as opposed to the fragmentary “gene-space” assemblies typical of modern projects using high-throughput methods and associated software. Approximately 99% of reads from the Illumina genomic libraries were accounted for, and nonplaceholder chromosomal sequence covers ∼94% of the optical map. Because no automated pipeline was found able to achieve the desired quality, methods are described in SI Appendix, SI Text.

                Genes of viral origin in the Fopius arisanus genome

                  *Fopius arisanus* (Sonan) is a braconid wasp (subfamily Opiinae) and biological control agent of a broad range of tephritid fruit fly species, including the global pests Mediterranean fruit fly *Ceratitis capitata* and the Oriental fruit fly *Bactrocera dorsalis*. In an effort to create foundational genomic resources for this species, the complete genome and transcriptomes for several wasp life stages have been recently generated. Manual annotation of 55 viral genes and phylogenetic analysis revealed that *F. arisanus* has independently acquired a symbiotic virus related to alpha-nudiviruses.

                  GenSAS v5.1: A Web-Based Platform for Structural and Functional Annotation and Curation of Genomes

                    The Genome Sequence Annotation Server (GenSAS) is an online platform that provides a pipeline for whole genome structural and functional annotation. Users can upload genome sequences and select from a variety of tools for repeat masking, prediction of gene models and other structural features as well as functional annotation tools. GenSAS integrates with JBrowse and Apollo to provide visualization and editing.