Data from: Discovery of MicroRNAs of the Stable Fly (Diptera: Muscidae) by High-Throughput Sequencing

This dataset reports discovery and initial comparative analysis of 88 presumptive microRNA (miRNA) sequences from the stable fly, obtained using high-throughput sequencing of small RNAs. The majority of stable fly miRNAs were 22-23 nucleotides (nt) in length. Many miRNAs were arthropod specific, and several mature miRNA sequences showed greater sequence identity to miRNAs from other blood-feeding dipterans such as mosquitoes rather than to Drosophilids. This initial step in characterizing the stable fly microRNAome provides a basis for further analyses of life stage-specific and tissue-specific expression to elucidate their functional roles in stable fly biology.

Veterinary Pest Genomics Center program

Data from: Pyrosequencing-Based Analysis of the Microbiome Associated with the Horn Fly, Haematobia irritans

The bacterial 16S tag-encoded FLX-titanium amplicon pyrosequencing (bTEFAP) method was used to carry out the classification analysis of bacterial flora in adult female and male horn flies and horn fly eggs. The bTEFAP method identified 16S rDNA sequences in our samples which allowed the identification of various prokaryotic taxa associated with the life stage examined. This is the first comprehensive report of bacterial flora associated with the horn fly using a culture-independent method. Several rumen, environmental, symbiotic and pathogenic bacteria associated with the horn fly were identified and quantified. This is the first report of the presence of Wolbachia in horn flies of USA origin and is the first report of the presence of Rikenella in an obligatory blood feeding insect.

Veterinary Pest Genomics Center program

Annotated reference transcriptome for female Culicoides sonorensis biting midges

Unigene sequences were annotated by BlastX alignment to the non-redundant protein database (National Center for Biotechnology Information/GenBank) and the Aedes aegypti and Culex quinquefasciatus gene annotations (Vectorbase). This was done with a 1e-05 expectation value. Top hits are shown including accession numbers and description, if available. Unigene number and corresponding GenBank accession numbers are provided for all C. sonorensis genes.

Veterinary Pest Genomics Center program