The bacterial 16S tag-encoded FLX-titanium amplicon pyrosequencing (bTEFAP) method was used to carry out the classification analysis of bacterial flora in adult female and male horn flies and horn fly eggs. The bTEFAP method identified 16S rDNA sequences in our samples which allowed the identification of various prokaryotic taxa associated with the life stage examined. This is the first comprehensive report of bacterial flora associated with the horn fly using a culture-independent method. Several rumen, environmental, symbiotic and pathogenic bacteria associated with the horn fly were identified and quantified. This is the first report of the presence of Wolbachia in horn flies of USA origin and is the first report of the presence of Rikenella in an obligatory blood feeding insect.
Veterinary Pest Genomics Center Overview
The Veterinary Pest Genomics Center (VPGC) is an initiative within the US Department of Agriculture's Agricultural Research Service (ARS). The vision for this initiative is to leverage big data solutions to evaluate risk from, and develop mitigations for invasive and other economically important veterinary pests. The introduction of invasive veterinary pests is accelerated by global change, including anomalies related to climate variability. An important aspect of this effort is to foster an innovation ecosystem involving the network of laboratories directly linked to ARS National Program 104 (Veterinary, Medical, and Urban Entomology), and related locations, in a way that allows ARS to leverage its scientific talent and other research assets.
VPGC's mission is to:
- Utilize key biological resources, next generation sequencing technology, and state-of-the-art bioinformatics approaches to sequence and annotate the genomes, transcriptomes, proteomes and metagenomes of important and emerging arthropod pests of veterinary importance
- Develop and use molecular tools for population genomics studies of veterinary pests in their indigenous and invasive ranges to understand the role of different evolutionary forces in shaping phenotypic variation of high-consequence to agriculture
- Apply biogeographic, spatial, and temporal analyses to quantify and predict economically important or potential veterinary pest distributional changes, and integrate these analyses with genetic studies of rapid evolution and adaptation of pests to new or changing environments
- Conduct feasibility studies to evaluate advanced computing hardware and software systems for their ability to store and analyze large data sets on veterinary pests, and the capacity to integrate results from longitudinal
Attribution info for the feature banner image: Tick larvae Creative Commons CC0 by Jason Tidwell
More about the program
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Data from: Pyrosequencing-Based Analysis of the Microbiome Associated with the Horn Fly, Haematobia irritans
This study reports the assembly of 4149 transcripts (≥200 nt) from testes dissected from New World Screwworm Cochliomyia hominivorax males obtained from the J06 strain used in the screwworm production plant in Pacora, Panama.